Host Plant Compatibility Shapes the Proteogenome of Frankia coriariae.
Host Plant Compatibility Shapes the Proteogenome of Frankia coriariae.
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DOI：
10.3389/fmicb.2017.00720
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发表时间：
2017
影响因子：
5.2
通讯作者：
Gtari M
中科院分区：
文献类型：
--
作者：
Ktari A;Gueddou A;Nouioui I;Miotello G;Sarkar I;Ghodhbane-Gtari F;Sen A;Armengaud J;Gtari M
在放线菌根际的分子信号网络选择主机兼容的Frankia菌株，触发感染过程，并最终固氮根瘤的发生。所涉及的分子触发因素仍然难以确定。根分泌物是一种高度动态的基质，在根际微生物组的建立中起着关键作用。已知RE诱导根瘤菌分泌结瘤因子、多糖和在豆科植物共生的情况下的其它蛋白质。利用下一代蛋白质组学方法，研究了亲和寄主（马桑）、不亲和寄主（赤杨）和非放线菌寄主（甜瓜梅洛）的RE处理革兰氏阴性杆菌BMG 5.1后，与宿主植物刺激物第一步识别相匹配的关键细菌信号。Frankia菌蛋白质组动态主要受宿主相容性的影响。代谢和信号转导是BMG 5.1在不同RE条件下的主要活性。第二组蛋白仅由C. Myrtifolia RE的表达主要与细胞壁重塑、信号转导和寄主信号处理活动有关。这些蛋白质可能足迹早期步骤接受识别主机的刺激之前，随后的事件的共生招聘。
Molecular signaling networks in the actinorhizal rhizosphere select host-compatible Frankia strains, trigger the infection process and eventually the genesis of nitrogen-fixing nodules. The molecular triggers involved remain difficult to ascertain. Root exudates (RE) are highly dynamic substrates that play key roles in establishing the rhizosphere microbiome. RE are known to induce the secretion by rhizobia of Nod factors, polysaccharides, and other proteins in the case of legume symbiosis. Next-generation proteomic approach was here used to decipher the key bacterial signals matching the first-step recognition of host plant stimuli upon treatment of Frankia coriariae strain BMG5.1 with RE derived from compatible (Coriaria myrtifolia), incompatible (Alnus glutinosa), and non-actinorhizal (Cucumis melo) host plants. The Frankia proteome dynamics were mainly driven by host compatibility. Both metabolism and signal transduction were the dominant activities for BMG5.1 under the different RE conditions tested. A second set of proteins that were solely induced by C. myrtifolia RE and were mainly linked to cell wall remodeling, signal transduction and host signal processing activities. These proteins may footprint early steps in receptive recognition of host stimuli before subsequent events of symbiotic recruitment.