Expression of Thy 1.2 surface antigen increases significantly during the murine mesenchymal stem cells cultivation period
Expression of Thy 1.2 surface antigen increases significantly during the murine mesenchymal stem cells cultivation period
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DOI：
10.1111/j.1440-169x.2007.00932.x
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发表时间：
2007-05-01
影响因子：
2.5
通讯作者：
Hosseini, Reza Hajji
中科院分区：
文献类型：
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作者：
Eslaminejad, Mohamadreza Baghaban;Nadri, Samad;Hosseini, Reza Hajji
本研究旨在研究小鼠间充质干细胞（mMSCs）在原代培养至第3代（相当于约15或16个群体倍增数）的培养期间表面抗原的缺失或表达。为此，将6-8周龄小鼠（NMRI或Balb/c）的骨髓细胞在75-cm（2）培养瓶中连续培养三代，在每代中使用流式细胞术检查培养物中CD 135、CD 44、CD 31、Thy1.2、CD 11b、CD 45、CD 34、Vcam 1、Sca-1和c-Kit抗原的表达。来自每个菌株的第3代细胞可以容易地分化成骨和脂肪，这表明它们的间充质性质。我们的结果表明，对于每种给定的抗原，表达该抗原的细胞的百分比已被改变的传代培养。统计学分析表明，传代之间几乎所有差异均具有统计学显著性。在这一时期，Thy 1.2的表达变化似乎是非常显著的，在第3代表达增加到整个群体的约一半。结论：该抗原可作为骨髓间充质干细胞的富集抗原。
This study sought to investigate the absence or expression of some surface antigens on murine mesenchymal stem cells (mMSCs) during the cultivation period of primary culture to passage 3 (equivalent to about 15 or 16 population doubling number). For this purpose, bone marrow cells from 6-8-week-old mice (either NMRI or Balb/c) were cultivated in 75-cm(2) culture flask for three successive passages, in each of which the culture was examined for the expression of CD135, CD44, CD31, Thy1.2, CD11b, CD45, CD34, Vcam1, Sca-1, and c-Kit antigens, using flow cytometry. Passage-3 cells from each strain can easily be differentiated into bone and fat, which was indicative of their mesenchymal nature. Our results demonstrated that for each given antigen, the percentages of the cells expressing that antigen had been changed by subcultures. The statistical analysis showed that nearly all differences between the passages were statistically significant. In this term, the expressional changes of Thy 1.2 seemed to be very significant in such a way that the expression increased to about half of the whole population in passage 3. In conclusion, it seems that this antigen could be considered as an enriching antigen for mMSCs population from bone marrow adherent cell culture.