Expression of inhibin/activin subunits and follistatin messenger ribonucleic acids and proteins in ovarian follicles and the corpus luteum during the human menstrual cycle.
Expression of inhibin/activin subunits and follistatin messenger ribonucleic acids and proteins in ovarian follicles and the corpus luteum during the human menstrual cycle.
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DOI：
10.1210/jcem.77.5.8077341
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发表时间：
1993-11
期刊：
影响因子：
--
通讯作者：
V J Roberts;S. Barth;A. El-Roeiy;S S Yen-S
中科院分区：
文献类型：
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作者：
V J Roberts;S. Barth;A. El-Roeiy;S S Yen-S
卵巢内激活素（β/β）和α/β二聚体在人类中的生理作用尚不清楚。卵泡抑素作为β亚基特异性高亲和力结合蛋白的鉴定增加了解释这些卵巢肽功能的体外研究的复杂性。因此，我们试图确定在体内房室分布的基因表达和蛋白定位的卵泡抑素同时从大量的人类卵巢中获得的卵泡和黄体中的卵泡素抑制素和激活素亚基（α，β A和β B）。用原位杂交和免疫组织化学方法检测卵泡抑素和卵泡抑素基因及其产物蛋白的表达。发现小窦卵泡（1-8 mm）的颗粒细胞表达α-、β A-和β B-亚基以及卵泡抑素的mRNA，并且最强的信号位于卵丘颗粒细胞中。在膜细胞层中，仅检测到α亚基mRNA。蛋白质被定位在对应于它们的mRNA的细胞隔室中，但此外，在没有基因表达的情况下，在卵泡膜细胞层中检测到β A亚基和卵泡抑素的蛋白质，这一观察结果与旁分泌作用相容。因此，小窦卵泡的颗粒细胞有潜力形成所有的二聚体的卵泡抑素和激活素，其自分泌和旁分泌的行动可能是由卵泡抑素在颗粒细胞和卵泡膜细胞层调制。随着优势卵泡的发育，亚基基因表达发生了显着的开关; β B亚基mRNA在任何细胞类型中都不再检测到，β A亚基表达出现在卵泡膜细胞中，沿着颗粒细胞中β A亚基和卵泡抑素的持续丰富表达。仅在颗粒细胞中发现与其mRNA相对应的蛋白质。在黄体中，卵泡抑素/激活素α和β A亚基和卵泡抑素mRNA和蛋白质仅在黄素化颗粒细胞中表达。黄体化的卵泡膜细胞缺乏可检测的mRNA信息或蛋白质。因此，黄体中的卵泡抑素-激活素-卵泡抑素系统似乎以自分泌方式起作用。（400字处截断摘要）
The physiological role of intraovarian activin (beta/beta) and inhibin (alpha/beta) dimers in humans in unclear. The identification of follistatin as a beta-subunit-specific high affinity binding protein has added complexities for the interpretation of in vitro studies concerning the functionalities of these ovarian peptides. We, therefore, have attempted to define in vivo compartmental distributions of gene expression and protein localization for inhibin and activin subunits (alpha, beta A, and beta B) concurrent with follistatin in ovarian follicles and corpus lutea obtained from a large number of human ovaries. In situ hybridization and immunohistochemistry were used for detecting the expression of genes encoding inhibin/activin subunits and follistatin and their gene products, the proteins. Granulosa cells of small antral follicles (1-8 mm) were found to express mRNA for alpha-, beta A-, and beta B-subunits as well as follistatin, and the strongest signals were localized in the cumulus granulosa cells. In the thecal cell layer, only alpha-subunit mRNA was detected. Proteins were localized in cellular compartments corresponding to their mRNA, but in addition, proteins for beta A-subunit and follistatin were detected in the thecal cell layers in the absence of gene expression, an observation compatible with a paracrine action. Thus, granulosa cells of the small antral follicle have the potential to form all dimers of inhibin and activin, and their autocrine and paracrine actions may be modulated by follistatin in both granulosa cell and thecal cell layers. With the development of a dominant follicle, remarkable switches in subunit gene expressions occurred; beta B-subunit mRNA was no longer detectable in any cell type, and beta A-subunit expression emerged in the thecal cells along with continued abundant expression of beta A-subunit and follistatin in the granulosa cells. Proteins were found only in granulosa cells corresponding to their mRNAs. In the corpus luteum, the inhibin/activin alpha- and beta A-subunits and follistatin mRNA and proteins were expressed exclusively in the luteinized granulosa cells. Luteinized thecal cells were devoid of detectable mRNA message or proteins. Thus, the inhibin-activin-follistatin system in the corpus luteum appears to function in an autocrine fashion.(ABSTRACT TRUNCATED AT 400 WORDS)