Measurement of cellular forces at focal adhesions using elastic micro-patterned substrates
Measurement of cellular forces at focal adhesions using elastic micro-patterned substrates
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DOI：
10.1016/s0928-4931(02)00309-0
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发表时间：
2003-03-03
期刊：
影响因子：
--
通讯作者：
Geiger, B
中科院分区：
文献类型：
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作者：
Schwarz, US;Balaban, NQ;Geiger, B
已知机械力在细胞行为调节中发挥重要作用，包括粘附、运动、分化和增殖。对于成纤维细胞等静止的机械活性细胞，对平坦基质的粘附主要发生在粘着斑部位，粘着斑是质膜上微米大小的蛋白质聚集体，其在细胞质侧连接到肌动蛋白细胞骨架。近年来，越来越多的证据表明粘着斑可作为机械传感器，将机械力转化为生化信号。我们通过一种新方法研究了粘着斑处的力和聚集之间的关系，该方法结合了弹性微图案基底（记录基底变形）、粘着斑蛋白的荧光标记（用于监测聚集）和线弹性理论反问题的数值解（用于计算粘着斑处的力）。我们发现力与聚集体的横向尺寸呈线性相关，应力常数为 5.5 nN/mum(2)。这一发现表明机械传感涉及聚集的调节。 (C) 2002 Elsevier Science B.V. 保留所有权利。
Mechanical force is known to play an important role in the regulation of cellular behaviour, including adhesion, motility, differentiation and proliferation. For stationary, mechanically active cells like fibroblasts, adhesion to flat substrates occurs mainly at sites of focal adhesions, which are micron-sized protein aggregates at the plasma membrane, which on the cytoplasmic side connect to the actin cytoskeleton. In recent years, evidence has been growing that focal adhesions act as mechanosensors which convert mechanical force into biochemical signalling. We have investigated the relationship between force and aggregation at focal adhesions by a new method which combines elastic micro-patterned substrates (to record substrate deformation), fluorescence labelling of focal adhesion proteins (to monitor aggregation) and numerical solution of the inverse problem of linear elasticity theory (to calculate forces at focal adhesions). We have found that force correlates linearly with lateral size of aggregation, with a stress constant of 5.5 nN/mum(2). This finding indicates that mechanosensing involves regulation of aggregation. (C) 2002 Elsevier Science B.V. All rights reserved.