TOWARDS THE PHENOTYPING OF SOFT-TISSUE TUMORS BY CELL-SURFACE MOLECULES
TOWARDS THE PHENOTYPING OF SOFT-TISSUE TUMORS BY CELL-SURFACE MOLECULES
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DOI：
10.1007/bf01600148
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发表时间：
1991-01-01
期刊：
影响因子：
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通讯作者：
MECHTERSHEIMER, G
中科院分区：
文献类型：
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作者：
MECHTERSHEIMER, G
本研究旨在通过细胞表面分子表征软组织肿瘤（STT）。为了实现这一点，对正常间充质组织的白细胞分化（CD）抗原和HLA分子的表达进行了广泛的检查。在STT中最终检查的抗原组包括CD 10、CD 13、CD 24、CD 34、CD 36、CD 56、CD 57、HLA-A、B、C、β-2-微球蛋白、HLA-DR、-DP和-DQ以及HLA-D相关不变链（Ii）。STT采用常规组织形态学和免疫组化标准。免疫组织学分析基于连续冷冻切片，其中之一用于显示CD 53抗原。这种非常广泛分布的白细胞/组织细胞限制性抗原允许间质“基质”细胞和肿瘤群体的背景之间的区别。在一些STT中，细胞表面分子的表达模式与其非肿瘤性对应物中的表达模式相对应。然而，大多数STT的细胞表面免疫表型与其来源细胞相比发生了相当大的变化。这些变化主要包括异常诱导/新表达，以及细胞表面抗原的异常下调/丢失（程度小得多）。然而，一些免疫表型配置描述，就目前而言，可以被认为是有用的补充，在这类复杂的肿瘤的鉴别诊断。这些数据还表明在间充质细胞的肿瘤转化过程中发生的细胞表面抗原表达的相当大的变化。详细分析肿瘤间充质细胞功能库的改变可能为STT的生物学提供新的见解，可能导致新的治疗干预概念。
This study is aimed at the characterization of soft tissue tumours (STT) by means of cell surface molecules. To achieve this, normal mesenchymal tissues were extensively examined for expression of leucocyte differentiation (CD) antigens and HLA molecules. The panel of antigens finally examined in STT comprised CD10, CD13, CD24, CD34, CD36, CD56, CD57, HLA-A,B,C, beta-2-microglobulin, HLA-DR, -DP, and -DQ and the HLA-D-associated invariant chain (Ii). STT were determined by conventional histomorphological and immunohistochemical criteria. The immunohistological analysis was based on serial frozen sections, one of which was used to demonstrate CD53 antigen. This very broadly distributed leuco/histiocyte-restricted antigen allowed for the distinction between the background of interstitial "stromal" cells and the neoplastic population. In some STT, the expression pattern of the cell surface molecules corresponded to that in their non-neoplastic counterparts. The majority of STT, however, showed considerable changes in the cell surface immunophenotype compared to their cells of origin. These alterations consisted mainly in an aberrant induction/neoexpression and, to a much lesser extent, in an aberrant down-regulation/loss of cell surface antigens. Nevertheless, some immunophenotype configurations are described which, for the time being, can be considered to be useful supplements in the differential diagnosis of this complex class of tumours. The data also indicate considerable changes in cell surface antigen expression occurring in the course of neoplastic transformation of mesenchymal cells. Detailed analysis of alterations in the functional repertoire of neoplastic mesenchymal cells might provide new insights into the biology of STT, possibly leading to new concepts for therapeutic intervention.