Expressions of COX-2 and VEGF-C in gastric cancer: correlations with lymphangiogenesis and prognostic implications
Expressions of COX-2 and VEGF-C in gastric cancer: correlations with lymphangiogenesis and prognostic implications
复制标题
DOI：
10.1186/1756-9966-30-14
复制
发表时间：
2011-01-28
影响因子：
11.3
通讯作者：
Hou, Mei
中科院分区：
文献类型：
--
作者：
Gou, Hong-Feng;Chen, Xin-Chuan;Hou, Mei
背景：环氧化酶-2 （COX-2）最近被认为通过上调血管内皮生长因子- c （VEGF-C）在乳腺癌和肺癌中促进淋巴管生成。然而，COX-2对胃癌淋巴管生成的影响尚不清楚。本研究旨在检测COX-2和VEGF-C在人胃癌中的表达情况，并分析其与淋巴管密度（LVD）、临床病理特征及生存预后的相关性。方法：采用免疫组化方法分析56例r0切除的原发性胃腺癌组织中COX-2、VEGF-C及LVD水平，同时收集25例同期患者的癌旁正常粘膜组织作为对照。分析COX-2、VEGF-C表达与LVD、临床病理参数的关系。通过单因素检验和多因素Cox回归评估Cox -2、VEGF-C和LVD水平与患者预后的相关性。结果：COX-2和VEGF-C在胃癌组织中的表达率分别为69.64%和55.36%。瘤周LVD明显高于正常组织和瘤内组织（P < 0.05）。与淋巴结转移及浸润深度有显著相关性（P = 0.003, P = 0.05）。VEGF-C与肿瘤周围LVD显著相关（r = 0.308, P = 0.021）。COX-2与VEGF-C （r = 0.110, P = 0.419）和LVD （r = 0.042, P = 0.758）无相关性。单因素分析显示，较高的COX-2表达和较高的肿瘤周围LVD会影响生存时间。多因素生存分析显示，年龄、COX-2表达和肿瘤周围低密度脂蛋白是独立的预后因素。结论：COX-2表达虽然与生存时间相关，但与VEGF-C和瘤周LVD无关。我们的数据没有显示COX-2的过表达通过上调胃癌中VEGF-C的表达来促进肿瘤淋巴管生成。年龄、COX-2和瘤周LVD是人胃癌的独立预后因素。
Background: Cyclooxygenase-2 (COX-2) has recently been considered to promote lymphangiogenesis by up-regulating vascular endothelial growth factor-C (VEGF-C) in breast and lung cancer. However, the impact of COX-2 on lymphangiogenesis of gastric cancer remains unclear. This study aims to test the expression of COX-2 and VEGF-C in human gastric cancer, and to analyze the correlation with lymphatic vessel density (LVD), clinicopathologic features and survival prognosis.Methods: Using immunohistochemistry, COX-2, VEGF-C and level of LVD were analyzed in 56 R0-resected primary gastric adenocarcinomas, while paracancerous normal mucosal tissues were also collected as control from 25 concurrent patients. The relationships among COX-2 and VEGF-C expression, LVD, and clinicopathologic parameters were analyzed. The correlations of COX-2, VEGF-C and level of LVD with patient prognosis were also evaluated by univariate tests and multivariate Cox regression.Results: The expression rates of COX-2 and VEGF-C were 69.64% and 55.36%, respectively, in gastric carcinoma. Peritumoral LVD was significantly higher than that in both normal and intratumoral tissue (P < 0.05). It was significantly correlated with lymph node metastasis and invasion depth (P = 0.003, P = 0.05). VEGF-C was significantly associated with peritumoral LVD (r = 0.308, P = 0.021). However, COX-2 was not correlated with VEGF-C (r = 0.110, P = 0.419) or LVD (r = 0.042, P = 0.758). Univariate analysis showed that survival time was impaired by higher COX-2 expression and higher peritumoral LVD. Multivariate survival analysis showed that age, COX-2 expression and peritumoral LVD were independent prognostic factors.Conclusions: Although COX-2 expression was associated with survival time, it was not correlated with VEGF-C and peritumoral LVD. Our data did not show that overexpression of COX-2 promotes tumor lymphangiogenesis through an up-regulation of VEGF-C expression in gastric carcinoma. Age, COX-2 and peritumoral LVD were independent prognostic factors for human gastric carcinoma.