RNA polymerase II holoenzyme recruitment is sufficient to remodel chromatin at the yeast PHO5 promoter
RNA polymerase II holoenzyme recruitment is sufficient to remodel chromatin at the yeast PHO5 promoter
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DOI：
10.1016/s0092-8674(00)80182-8
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发表时间：
1997-04-04
期刊：
影响因子：
64.5
通讯作者：
Horz, W
中科院分区：
文献类型：
--
作者：
Gaudreau, L;Schmid, A;Horz, W
我们通过融合蛋白检测酵母中PHO5启动子的转录激活和染色质重塑，融合蛋白被认为是在缺乏经典激活区的情况下通过将RNA聚合酶II全酶招募到DNA来发挥作用。这些杂合蛋白(例如，Gal11+Pho4或Gal4(58-97)+Pho4在存在GAL11P等位基因的情况下)有效地激活了转录并重塑了染色质。在缺失TATA的PHO5启动子上也观察到类似的染色质重塑，因此无法支持转录。我们的结论是，全酶或相关蛋白的重新募集足以进行染色质重塑。我们还表明，SWI/SNF复合体既不是野生型PHO5有效转录所必需的，也不是GAL1启动子有效转录所必需的，我们在没有Snf2的情况下观察到PHO5几乎完全的染色质重塑。
We examine transcriptional activation and chromatin remodeling at the PHO5 promoter in yeast by fusion proteins that are thought to act by recruiting the RNA polymerase II holoenzyme to DNA in the absence of a classic activating region. These hybrid proteins (e.g., Gal11+Pho4 or Gal4(58-97)+Pho4 in the presence of a GAL11P allele) efficiently activated transcription and remodeled chromatin. Similar chromatin remodeling was observed at a PHO5 promoter deleted for TATA and thus unable to support transcription. We conclude that recruitment of the holoenzyme or associated proteins suffices for chromatin remodeling. We also show that the SWI/SNF complex is required neither for efficient transcription of the wild-type PHO5 nor the GAL1 promoters, and we observe nearly complete chromatin remodeling at PHO5 in the absence of Snf2.