IDENTIFICATION AND PARTIAL CHARACTERIZATION OF THE SOYBEAN-DUST ALLERGENS INVOLVED IN THE BARCELONA ASTHMA EPIDEMIC
IDENTIFICATION AND PARTIAL CHARACTERIZATION OF THE SOYBEAN-DUST ALLERGENS INVOLVED IN THE BARCELONA ASTHMA EPIDEMIC
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DOI：
10.1016/0091-6749(90)90198-d
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发表时间：
1990-04-01
影响因子：
14.2
通讯作者：
REED, CE
中科院分区：
文献类型：
--
作者：
RODRIGO, MJ;MORELL, F;REED, CE
西班牙巴塞罗那的哮喘疫情被归因于港口卸货产生的粉尘。研究对象分为4组，每组10人：(1)在流行日因哮喘发作而到巴塞罗那急诊室就诊的患者，A组，(2)在非流行日因哮喘发作而到急诊室就诊的患者，B组，(3)来自其他城市的哮喘患者，C组，以及(4)年龄、性别与A组、D组匹配的巴塞罗那非哮喘患者。相比之下，在B组和C组各10名受试者中只有1名，在D组中没有一名受试者对未清洗的豆类和果壳提取物有IgE。放射免疫分析表明，在哮喘发作期间，哮喘患者的血清中主要与大豆壳和灰尘提取物发生反应。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳薄层等电聚焦显示蛋白条带为97.4~14.4kd，等电点为6~3.5。经Western印迹和薄层等电聚焦/免疫等电聚焦，哮喘发作期患者的IgE与分子量在42~21kd的两条蛋白质带呈弱反应，与分子量为14.4kd的糖蛋白条带和等电点lt；6呈强阳性反应。
Asthma epidemics in Barcelona, Spain, have been attributed to dust generated by the unloading of soybeans in the harbor. Sera of four different groups of 10 subjects in each group were studied: (1) patients attending an emergency room in Barcelona for an asthma attack on epidemic days, group A, (2) patients attending an emergency room for an attack on nonepidemic days, group B, (3) patients with asthma from other cites, group C, and (4) patients without asthma from Barcelona matched by age and sex with group A, group D. All subjects in group A had IgE to allergens in extracts of various soybean samples. In contrast, only one of the 10 subjects in each of groups B and C and none of those subjects in group D had IgE to uncleaned bean and hull extracts. Radioimmunoassay demonstrated that in sera from patients with asthma during an asthma outbreak reacted primarily to soybean hull and dust extracts. Sodium dodecyl sulfate-polyacrylamide and gel electrophoresis thin-layer isoelectrofocusing demonstrated protein bands of 97.4 to < 14.4 kd and isoelectric point between 6 and 3.5. By Western blot and thin layer isoelectrofocusing/blotted radioimmunoisoelectofocusing, IgE of patients with asthma during an asthma outbreak reacted weakly to two proteins bands of molecular weight ranging from 42 to 21 kd, strongly to glycoprotein bands with molecular weight < 14.4 kd, and isoelectric point < 6, which appeared to be the major allergens.