DIAGNOSIS OF HERPES-SIMPLEX ENCEPHALITIS - APPLICATION OF POLYMERASE CHAIN-REACTION TO CEREBROSPINAL-FLUID FROM BRAIN-BIOPSIED PATIENTS AND CORRELATION WITH DISEASE
DIAGNOSIS OF HERPES-SIMPLEX ENCEPHALITIS - APPLICATION OF POLYMERASE CHAIN-REACTION TO CEREBROSPINAL-FLUID FROM BRAIN-BIOPSIED PATIENTS AND CORRELATION WITH DISEASE
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DOI：
10.1093/infdis/171.4.857
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发表时间：
1995-04-01
影响因子：
6.4
通讯作者：
TILLES, J
中科院分区：
文献类型：
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作者：
LAKEMAN, FD;WHITLEY, RJ;TILLES, J
从脑组织活检后分离单纯疱疹病毒(HSV)已被认为是诊断单纯疱疹病毒性脑炎(HSE)的参考标准。在评估HSE抗病毒治疗的过程中，脑脊液(CSF)取自经诊断性脑活检的有HSE临床症状的患者。应用聚合酶链式反应(PCR)检测54例HSE患者中53例(98%)脑脊液中HSV DNA，18例HSE患者脑组织活检前采集的脑脊液标本中均检出HSV DNA。19份脑脊液标本中有4份在抗病毒治疗2周后呈阳性。在47例脑组织培养阴性的患者中，有3例(6%)阳性。脑脊液中单纯疱疹病毒DNA的检测与年龄和局部放射学表现显著相关。因此，单纯疱疹病毒DNA的聚合酶链式反应检测应作为诊断HSE的标准。
Isolation of herpes simplex virus (HSV) from brain tissue after biopsy has been considered the reference standard for the diagnosis of herpes simplex encephalitis (HSE). During the evaluation of antiviral treatment of HSE, cerebrospinal fluid (CSF) was obtained from patients with clinical disease indicative of HSE who underwent diagnostic brain biopsy. HSV DNA was detected by polymerase chain reaction (PCR) in CSF of 53 (98%) of 54 patients with biopsy-proven HSE and was detected in all 18 CSF specimens obtained before brain biopsy from patients with proven HSE. Four of 19 CSF specimens were positive after 2 weeks of antiviral therapy. Positive results were found in 3 (6%) of 47 patients whose brain tissue was culture-negative. Detection of HSV DNA in the CSF correlated significantly with age and focal radiographic findings. Thus, PCR detection of HSV DNA should be the standard for diagnosis of HSE.