Proteomics screening of adenosine triphosphate-interacting proteins in the liver of diazinon-treated rats
Proteomics screening of adenosine triphosphate-interacting proteins in the liver of diazinon-treated rats
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DOI：
10.1177/0960327115619771
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发表时间：
2016-10
影响因子：
2.8
通讯作者：
A. Pourtaji;R. Y. Robati;P. Lari;H. Hosseinzadeh;M. Ramezani;K. Abnous
中科院分区：
文献类型：
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作者：
A. Pourtaji;R. Y. Robati;P. Lari;H. Hosseinzadeh;M. Ramezani;K. Abnous
目的：二嗪农（DZN）是目前世界上用于农业害虫防治的重要有机磷农药之一。一些研究表明，暴露于DZN可能会改变肝脏中的蛋白质表达。为了进一步研究DZN的毒性机制，在亚急性毒素暴露后，在大鼠肝脏中分离和鉴定差异表达的ATP相互作用蛋白。主要方法：雄性大鼠随机分为对照组（玉米油组）和DZN组（15 mg/kg），每天灌胃1次，连续4周。肝组织均质化后，将裂解物孵育ATP-琼脂糖珠。几次洗涤后，ATP相互作用蛋白被洗脱并在2-D聚丙烯酰胺凝胶上分离。使用基质辅助激光解吸/离子化/飞行时间和Mascot数据库切割和鉴定Depletion表达的蛋白。通过进化关系蛋白质分析（PANTHER）网站，根据它们的生物学过程对鉴定的蛋白质进行分类。关键发现：在这项工作中，我们发现，几个关键蛋白参与的生物过程，如抗氧化系统，氧化应激，细胞凋亡和代谢的差异表达后，亚急性暴露于DZN。
Aim: Diazinon (DZN) is one of the most important organophosphorus compounds used to control pests in agriculture in many countries. Several studies have shown that exposure to DZN may alter protein expression in the liver. In order to further investigate the mechanism of DZN toxicity, differentially expressed ATP-interacting proteins, following subacute exposure to toxin, were separated and identified in rat liver. Main methods: Male rats were equally divided into four groups: control (corn oil) and DZN (15 mg/kg) by gavage once a day for 4 weeks. After homogenization of liver tissue, lysates were incubated ATP-sepharose beads. After several washes, ATP-interacting proteins were eluted and separated on 2-D polyacrylamide gels. Deferentially expressed proteins were cut and identified using matrix-assisted laser desorption/ionization/time-of-flight and Mascot database. Identified proteins were classified according to their biological process using protein analysis through evolutionary relationships (PANTHER) Web site. Key finding: In this work, we showed that several key proteins involved in biological processes such as antioxidant system, oxidative stress, apoptosis, and metabolism were differentially expressed after subacute exposure to DZN.