Extravascular, extracellular water in rabbit lung using Na and sucrose indicators.
Extravascular, extracellular water in rabbit lung using Na and sucrose indicators.
复制标题
使用钠和蔗糖指示剂测定兔肺中的血管外、细胞外水。
DOI：
10.1152/jappl.1982.52.3.792
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
CheneyJr,FW
中科院分区：
文献类型：
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作者：
Pavlin,DJ;Haschke,RH;Nessly,ML;CheneyJr,FW
我们通过稳态技术，使用钠和蔗糖指示剂比较了 8 只正常兔肺和 11 只水肿兔肺的肺血管外、细胞外水空间的体积。血管外细胞外空间 (EVECW) 的体积，以毫升每克干肺表示，由 24Na、[3H]蔗糖或总钠在 1) 通过匀浆肺离心获得的上清液或 2) 完整肺碎片中的分布体积确定。正常肺中测量的 EECW 无显着差异 [24Na 1.88 +/- (SD) 0.31； [3H]蔗糖1.84 +/- (SD) 0.27]或肺水肿[24Na 4.78 +/- (SD) 1.44； [ 3 H]蔗糖4.41 +/- (SD) 1.33]当比较均质肺的上清液时。同样，在正常肺的完整碎片中测量时，这两种同位素的分布体积 (EVECW) 之间没有显着差异 [24Na 2.08 +/- (SD) 0.28； [3H]蔗糖1.68+/-(SD)0.37]。上清液中测得的 EVECW 体积小于两种同位素碎片中的体积。洗涤离心的组织沉淀并将洗涤活性添加到初始上清液活性中产生与片段技术相同的结果。未能检测到肺中 24Na 和 [3H] 蔗糖空间之间的显着差异表明，细胞内钠对肺水测量的贡献很小且相对不显着，至少在本实验的范围内是这样。
We compared the volume of the pulmonary extravascular, extracellular water space using sodium and sucrose indicators in 8 normal and 11 edematous rabbit lungs by steady-state techniques. The volume of the extravascular, extracellular space (EVECW), expressed in milliliters per gram dry lung, was determined from the volume of distribution of 24Na, [3H]sucrose, or total sodium in 1) supernatant solutions obtained by centrifugation of homogenized lung or 2) intact lung fragments. There was no significant difference in EVECW measured in normal lungs [24Na 1.88 +/- (SD) 0.31; [3H]sucrose 1.84 +/- (SD) 0.27] or edematous lungs [24Na 4.78 +/- (SD) 1.44; [3H]sucrose 4.41 +/- (SD) 1.33] when comparing supernate solutions of homogenized lungs. Similarly, there was no significant difference between the volumes of distribution (EVECW) of these two isotopes when measured in intact fragments of normal lung [24Na 2.08 +/- (SD) 0.28; [3H]sucrose 1.68 +/- (SD) 0.37]. The volume of EVECW measured in supernates was less than in fragments with both isotopes. Washing the centrifuged tissue pellet and adding the wash activity to the initial supernate activity yielded results that were equal to the fragment technique. The failure to detect significant differences between 24Na and [3H]sucrose spaces in the lung suggests that the contribution on intracellular sodium to the measurement of lung water is small and relatively insignificant, at least within the confines of this experiment.