Genomic alterations associated with malignancy in head and neck cancer
Genomic alterations associated with malignancy in head and neck cancer
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DOI：
10.1002/(sici)1097-0347(199803)20:2
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发表时间：
1998-03-01
影响因子：
2.9
通讯作者：
Petersen, I
中科院分区：
文献类型：
--
作者：
Bockmuhl, U;Wolf, G;Petersen, I
背景。比较基因组杂交（CGH）对50例原发性头颈部鳞状细胞癌（HNSCC）进行了比较基因组杂交（CGH），以发现这些肿瘤进展的分子遗传改变。在CGH中，等量的不同标记的肿瘤脱氧核糖核酸（DNA）和正常参考DNA同时与正常中期染色体杂交。它们通过不同的荧光染料可视化，信号强度分别定量为沿单个染色体的灰度水平。通过比值图像和平均比值谱的计算，确定了代表性过高和代表性不足的DNA片段。普遍观察到变化超过50%的染色体1 p的HNSCC包括删除,4,5,6,8 p, 9 p, 11日13问,18 q, 11和21 q和DNA传播的问题以及3 q, 8 q, 16 p, q, 17 19日20问,22 q比率的计算资料显示高度分化的癌肿瘤的子组(G1)是由染色体3 p的删除,5 q,和90一起群体中3 q,暗示与早期肿瘤的发展。因此，未分化肿瘤（G3）的特征是染色体4q、8p、11q、13q、18q、21q的额外缺失，以及Ip、11q13、19和22q的过度表达。我们的数据表明，染色体失衡的CGH模式可能有助于确定头颈部鳞状细胞癌的恶性潜能。(C) 1998 John Wiley & Sons公司
Background. Comparative genomic hybridization (CGH) was performed on 50 primary head and neck squamous cell carcinomas (HNSCC) to discover molecular genetic alterations underlying the progression of these tumors.Methods. In CGH, equal amounts of differently labeled tumor deoxyribonucleic acid (DNA) and normal reference DNA were hybridized simultaneously to normal metaphase chromosomes. They were visualized by different fluorochromes, and the signal intensities were quantitated separately as gray levels along the single chromosomes. The over-and underrepresented DNA segments were determined by computation of ratio images and average ratio profiles.Results. Prevalent changes observed in more than 50% of the HNSCC included deletions of chromosomes 1p, 4, 5q, 6q, 8p, 9p, 11, 13q, 18q, and 21q and DNA overrepresentations of 11q13 as well as 3q, 8q, 16p, 17q, 19, 20q, and 22q, The calculation of ratio profiles of tumor subgroups revealed that well differentiated carcinomas (G1) were defined by the deletions of chromosomes 3p, 5q, and 90 together with the overrepresentation of 3q, suggesting the association with early tumor development. Accordingly, the undifferentiated tumors (G3) were characterized by additional deletions of chromosomes 4q, 8p, 11q, 13q, 18q, 21q, and overrepresentations of Ip, 11q13, 19, and 22q.Conclusion. Our data indicate that the CGH patterns of chromosomal imbalances may help to define the malignant potential of head and neck squamous cell carcinomas. (C) 1998 John Wiley & Sons, Inc.