Determination of the major tautomeric form of the covalently modified adenine in the (+)-CC-1065-DNA adduct by 1H and 15N NMR studies.
Determination of the major tautomeric form of the covalently modified adenine in the (+)-CC-1065-DNA adduct by 1H and 15N NMR studies.
复制标题
通过 1H 和 15N NMR 研究测定 ( )-CC-1065-DNA 加合物中共价修饰腺嘌呤的主要互变异构形式。
DOI：
10.1021/bi00493a002
复制
发表时间：
1990
期刊：
影响因子：
2.9
通讯作者：
Hurley,LH
中科院分区：
文献类型：
--
作者：
Lin,CH;Hurley,LH
材料和方法化学品。（+）-CC-1065得自The Upjohn Co.，不经进一步纯化即使用。[15N]用于制备NMR缓冲液的试剂、磷酸钠（99.99%）和氯化钠（99.99%）购自Aldrich Co.。HPLC水和甲醇购自巴克斯特科学和费舍尔公司，分别羟基磷灰石用于纯化12-mer双链体和12-mer加合物。
Materials and MethodsChemicals.(+)-CC-1065 was obtained from The Upjohn Co. and used without further purification.[15N] Benzamide (99.5 atom% l5N) was purchased from MSD Isotopes Co. Reagents used to prepare NMR buffer, sodium phosphate (99.99%), and sodium chloride (99.99%) were purchased from Aldrich Co. HPLC water and methanol were purchased from Baxter Scientific and Fisher Co., respectively. Hydroxylapatite used to purify the 12-mer duplex and the 12-mer adduct was