Identification of zinc finger binding protein 89 (ZBP-89) as a transcriptional activator for a major bovine growth hormone receptor promoter.
Identification of zinc finger binding protein 89 (ZBP-89) as a transcriptional activator for a major bovine growth hormone receptor promoter.
复制标题
鉴定锌指结合蛋白 89 (ZBP-89) 作为主要牛生长激素受体启动子的转录激活剂。
DOI：
10.1016/j.mce.2006.03.001
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发表时间：
2006
影响因子：
4.1
通讯作者：
Jiang,Honglin
中科院分区：
文献类型：
--
作者：
Xu,Qingfu;Springer,Lynn;Merchant,JuanitaL;Jiang,Honglin
本研究的目的是确定调节生长激素受体（GHR） 1A mRNA表达的转录因子，这是牛肝脏中主要的GHR mRNA变体。脱氧核糖核酸酶I足迹分析显示，GHR1A启动子区−69至−30（相对于GHR1A mRNA的转录起始位点）含有牛肝核蛋白的结合位点。通过酵母单杂交分析，锌指结合蛋白89 （ZBP-89）被鉴定为该启动子区域的结合蛋白。ZBP-89与GHR1A启动子区−69至−30的结合通过电泳迁移量转移试验进一步证实。在共转染分析中，ZBP-89的过表达增强了GHR1A启动子的活性（P<0.01），这种增强依赖于启动子中ZBP-89的结合位点。这些结果表明ZBP-89是调节GHR1A mRNA表达的转录因子。
The objective of this study was to identify the transcription factors that regulate the expression of growth hormone receptor (GHR) 1A mRNA, a major GHR mRNA variant in the bovine liver. A deoxyribonuclease I footprint analysis revealed that the GHR1A promoter region −69 to −30 (relative to the transcription start site for GHR1A mRNA) contained binding sites for bovine liver nuclear proteins. Using a yeast one-hybrid analysis, zinc finger binding protein 89 (ZBP-89) was identified as a binding protein to this promoter region. Binding of ZBP-89 to the GHR1A promoter region −69 to −30 was further confirmed by an electrophoretic mobility shift assay. In cotransfection analyses, overexpression of ZBP-89 enhanced (P<0.01) the activity of the GHR1A promoter and this enhancement was dependent on the putative ZBP-89 binding site in the promoter. These results together indicate that ZBP-89 is a transcription factor that regulates the expression of GHR1A mRNA.