Histological Changes of the Testicular Interstitium during Postnatal Development in Microminipigs
Histological Changes of the Testicular Interstitium during Postnatal Development in Microminipigs
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DOI：
10.1177/0192623319827477
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发表时间：
2019-06-01
影响因子：
1.5
通讯作者：
Shibata, Masatoshi
中科院分区：
文献类型：
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作者：
Kangawa, Akihisa;Otake, Masayoshi;Shibata, Masatoshi
微型猪因其易于管理的体型而成为毒理学和药理学相关研究中有吸引力的动物模型。在这项研究中，研究了 0 至 12 个月龄微型猪的睾丸间质发育和类固醇生成。睾丸间质主要由两种间质细胞（大间质细胞和小间质细胞）和少量巨噬细胞和肥大细胞组成。在各个年龄段的肾小管周围区域均观察到大的间质细胞。小Leydig细胞在幼年时存在于小叶间和被膜下区域，然后逐渐转化为大Leydig细胞。睾丸间质细胞的组成在 3 个月大、精子发生完成后开始增加，并在 12 个月大时达到约 35%。从 0 个月大起，通过免疫组织化学检测 Leydig 细胞中的类固醇生成酶。血清睾酮水平从 1.5 月龄到 4.5 月龄大幅增加，这与之前报道的微型猪的性发育年龄非常吻合。由于不同物种间睾丸间质存在显着差异，本研究获得的基本信息将为今后所有微型猪的毒性评估提供有用的参考。
Microminipigs have become an attractive animal model for the toxicology- and pharmacology-related studies because of their manageable size. In this study, the development of the testicular interstitium and steroidogenesis in microminipigs, from 0 to 12 months of age, were investigated. Testicular interstitium was mostly composed of two types of Leydig cells (large and small Leydig cells) and a few macrophages and mast cells. Large Leydig cells were observed in the peritubular area throughout all the ages. Small Leydig cells were present in the interlobular and subcapsular areas at an early age and then gradually converted into large Leydig cells. Testicular composition of the Leydig cells began to increase after 3 months of age, when spermatogenesis was completed, and reached approximately 35% at 12 months. Steroidogenic enzymes in Leydig cells were detected by immunohistochemistry from 0 month of age. Serum testosterone levels increased substantially from 1.5 to 4.5 months of age, which coincided well with the age of sexual development previously reported in microminipigs. Because the interstitial space of the testis has dramatic variations between species, the basic information obtained in the present study will be a useful reference for all the future toxicity evaluations in microminipigs.