Immunoregulation of Macrophages by Controlling Winding and Unwinding of Nanohelical Ligands
Immunoregulation of Macrophages by Controlling Winding and Unwinding of Nanohelical Ligands
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DOI：
10.1002/adfm.202103409
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发表时间：
2021-06-18
影响因子：
19
通讯作者：
Kang, Heemin
中科院分区：
文献类型：
--
作者：
Bae, Gunhyu;Jeon, Yoo Sang;Kang, Heemin
开发具有改变其先天特征的能力的材料可以帮助解开细胞和配体显示特征之间的直接相互作用。本研究展示了将展示细胞粘附性Arg-Gly-Asp（RGD）配体的磁性纳米螺旋部分接枝到材料表面。这些使得能够纳米级控制其未接枝部分的快速缠绕（“W”）和解绕（“UW”），例如通过改变永磁体的位置同时保持配体缀合的纳米螺旋表面积恒定来改变纳米螺旋解绕的方向（下、中和上方向）。解旋（“UW”）设置细胞相容性促进直接整联蛋白募集到配体缀合的纳米螺旋上，以介导巨噬细胞的桩蛋白粘附组装体的发展，所述巨噬细胞的桩蛋白粘附组装体分别在单细胞水平上使用玻璃和硅基底在体外和体内设置下刺激M2极化。真实的时间和体内成像表明，纳米螺旋表现出可逆的解旋，缠绕和解旋设置，其调节巨噬细胞的时间分辨粘附和极化。据设想，这种远程的，可逆的，和细胞相容性的控制可以帮助阐明分子水平的细胞材料的相互作用，调节再生/抗炎免疫反应的植入物。
Developing materials with the capability of changing their innate features can help to unravel direct interactions between cells and ligand-displaying features. This study demonstrates the grafting of magnetic nanohelices displaying cell-adhesive Arg-Gly-Asp (RGD) ligand partly to a material surface. These enable nanoscale control of rapid winding ("W") and unwinding ("UW") of their nongrafted portion, such as directional changes in nanohelix unwinding (lower, middle, and upper directions) by changing the position of a permanent magnet while keeping the ligand-conjugated nanohelix surface area constant. The unwinding ("UW") setting cytocompatibility facilitates direct integrin recruitment onto the ligand-conjugated nanohelix to mediate the development of paxillin adhesion assemblies of macrophages that stimulate M2 polarization using glass and silicon substrates for in vitro and in vivo settings, respectively, at a single cell level. Real time and in vivo imaging are demonstrated that nanohelices exhibit reversible unwinding, winding, and unwinding settings, which modulate time-resolved adhesion and polarization of macrophages. It is envisaged that this remote, reversible, and cytocompatible control can help to elucidate molecular-level cell-material interactions that modulate regenerative/anti-inflammatory immune responses to implants.