Radical scavenging and anti-inflammatory activity of extracts from Opuntia humifusa Raf.
Radical scavenging and anti-inflammatory activity of extracts from Opuntia humifusa Raf.
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DOI：
10.1211/jpp.58.1.0014
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发表时间：
2006-01-01
影响因子：
3.3
通讯作者：
Rhee, MH
中科院分区：
文献类型：
--
作者：
Cho, JY;Park, SC;Rhee, MH
葎草。（O. humifusa Raf.）是仙人掌科的一员。为了确定仙人掌的抗氧化和抗炎作用，采用DPPH（2,2-二苯基-l-苦酰肼自由基）和黄嘌呤氧化酶测定方法，对仙人掌叶片的不同溶剂组分（甲醇、己烷、氯仿、乙酸乙酯、丁醇和水）和产生一氧化氮（NO）的巨噬细胞进行了测试。我们发现O. humifusa Raf。具有很强的抗氧化和抗炎活性。因此，除水层外，所有溶剂组分均表现出强大的清除作用。乙酸乙酯部位的清除率高于其他部位，IC50值分别为3.6和48.2 μ g/mL-1。根据活性引导分馏法，发现槲皮素在乙酸乙酯组分中具有清除自由基的活性。相比之下，只有两个部分（氯仿和乙酸乙酯）显著抑制脂多糖（LPS）激活的RAW264.7细胞产生一氧化氮。此外，氯仿和乙酸乙酯组分显著抑制了诱导型一氧化氮合成酶（iNOS）和白细胞介素-6 （IL-6）的表达。此外，乙酸乙酯组分显著阻断LPS刺激RAW264.7细胞IL-1 β的表达。因此，研究结果表明，O. humifusa Raf。可能通过直接清除活性和抑制活性物质的产生，以及调节炎症细胞因子的表达来调节自由基诱导的毒性。最后是O. humifusa Raf。可作为抗反应性物种介导疾病的功能性食品或药物。
Opuntia humifusa Raf. (O. humifusa Raf.) is a member of the Cactaceae family. To determine the antioxidative and anti-inflammatory effects of this herb, various solvent fractions (methanol, hexane, chloroform, ethyl acetate, butanol, and water) prepared from the leaves of cacti were tested using DPPH (2,2-diphenyl-l-picrylhydrazyl radical) and xanthine oxidase assays, and nitric oxide (NO)producing macrophage cells. We found that O. humifusa Raf. displayed potent antioxidative and anti-inflammatory activity. Thus, all solvent fractions, except for the water layer, showed potent scavenging effects. The scavenging effect of the ethyl acetate fraction was higher than that of the other fractions, with IC50 values of 3.6 and 48.2 mu g/mL-1. According to activity-guided fractionation, one of the active radical scavenging principles in the ethyl acetate fraction was found to be quercetin. In contrast, only two fractions (chloroform and ethyl acetate) significantly suppressed nitric oxide production from the lipopolysaccharide (LPS)-activated RAW264.7 cells. In addition, chloroform and ethyl acetate fractions significantly blocked the expression of inducible nitric oxide synthetase (iNOS) and interleukin-6 (IL-6) from the RAW264.7 cells stimulated by LIPS. Moreover, ethyl acetate fractions significantly blocked the expression of IL-1 beta from the RAW264.7 cells stimulated by LPS. Therefore, the results suggested that O. humifusa Raf. may modulate radical-induced toxicity via both direct scavenging activity and the inhibition of reactive species generation, and the modulation of the expression of inflammatory cytokines. Finally, O. humifusa Raf. may be useful as a functional food or drug against reactive species-mediated disease.