Rapid detection of a Schistosoma mansoni circulating antigen excreted in urine of infected individuals by using a monoclonal antibody
Rapid detection of a Schistosoma mansoni circulating antigen excreted in urine of infected individuals by using a monoclonal antibody
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DOI：
10.1128/jcm.37.2.354-357.1999
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发表时间：
1999-02-01
影响因子：
9.4
通讯作者：
Ezzat, F
中科院分区：
文献类型：
--
作者：
Attallah, AM;Ismail, H;Ezzat, F
血吸虫循环抗原用于指示感染强度并评估治愈情况。免疫球蛋白 G2a (IgG2a) 小鼠单克隆抗体用于快速点酶联免疫吸附测定 (ELISA；FDA)，可在现场快速、简单地诊断血吸虫病。七百名埃及人接受了曼氏血吸虫和其他寄生虫感染的寄生虫学检查。直肠活检作为粪便中没有曼氏链球菌卵的个体的“黄金标准”。通过加藤涂片法对 152 名粪便中含有虫卵的个体中仅 100 人进行了虫卵计数。通过 ELISA 评估血清中的特异性抗血吸虫 IgG 抗体。 FDA 对 700 人的尿液样本进行了循环抗原检测。该检测显示 433 名感染者的敏感性为 93%，267 名未感染者的特异性为 89%。与 ELISA 抗体检测效率（75%）和粪便分析（60%）相比，FDA 显示抗原检测效率最高（91%）。此外，FDA还检测到感染患者的粪便中含有20个虫卵。此外，不同临床阶段血吸虫病患者的样本中 FDA 的敏感性为 90% 至 94%。 96 个尿液样本的所有测定步骤可在室温下 30 分钟内完成。该单克隆抗体在曼氏血吸虫和埃及血吸虫的不同抗原提取物以及感染个体的尿液中鉴定出 74 kDa 抗原。此外，仅在尿液样本中鉴定出 30 kDa 的降解产物。根据这些结果，FDA 应作为血吸虫感染敏感且特异诊断的快速工具。
Schistosoma circulating antigens were used to indicate the infection intensity and to assess cure. An immunoglobulin G2a (IgG2a) mouse monoclonal antibody was used in a fast dot-enzyme-linked immunosorbent assay (ELISA; FDA) for rapid and simple diagnosis of schistosomiasis in the field. Seven hundred Egyptians were parasitologically examined for Schistosoma mansoni and other parasitic infections. A rectal biopsy was done as a "gold standard" for individuals showing no S. mansoni eggs in their feces. Egg counts were obtained by the Kato smear method for only 100 of 152 individuals with eggs in their feces. Specific anti-schistosome IgG antibodies were evaluated in sera by ELISA. Urine samples from the 700 individuals were tested by FDA for detection of the circulating antigen. The assay showed a sensitivity of 93% among 433 infected individuals and a specificity of 89% among 267 noninfected individuals. FDA showed the highest efficiency of antigen detection (91%) compared with the efficiency of antibody detection by ELISA (75%) and stool analysis (60%). In addition, FDA detected infected patients with 20 eggs/g of feces. Also, the sensitivity of FDA ranged from 90 to 94% among samples from patients with different clinical stages of schistosomiasis. All the assay steps can be completed within 30 min at room temperature for 96 urine samples. The monoclonal antibody identified a 74-kDa antigen in different antigenic extracts of S. mansoni and Schistosoma haematobium and in the urine of infected individuals. In addition, a 30-kDa degradation product was identified only in the urine samples. On the basis of these results, FDA should be used as a rapid tool for the sensitive and specific diagnosis of Schistosoma infection.