Induction of zinc-finger proliferation 1 expression in non-myelinating Schwann cells after denervation.
Induction of zinc-finger proliferation 1 expression in non-myelinating Schwann cells after denervation.
复制标题
去神经后非髓鞘雪旺细胞中锌指增殖1表达的诱导。
DOI：
10.1016/j.neuroscience.2008.02.078
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发表时间：
2008
期刊：
影响因子：
3.3
通讯作者：
Rimer,M
中科院分区：
文献类型：
--
作者：
Ellerton,EL;Thompson,WJ;Rimer,M
终末雪旺细胞(TSCs)是包裹神经肌肉连接处神经末梢的非髓鞘神经胶质细胞。它们是维持神经肌肉突触所必需的，并可能在神经损伤后突触连接的恢复中发挥重要作用。TSCs在神经损伤后获得一种反应性表型，其特征是细胞突起的延长，这可能有助于神经的再支配。支持TSC反应状态的分子信号事件仍然难以捉摸，尤其是对TSC激活过程中参与转录重编程的转录因子知之甚少。先前的研究表明，锌指转录因子家族中有9个成员参与了雪旺细胞(SC)的发育和髓鞘形成，据报道，在去神经支配的其他非髓鞘干细胞中，有一个这样的蛋白水平升高。我们推测锌指转录因子可能在TSC激活过程中发挥作用。由于其相对匮乏，TSCs很难从分子水平上进行研究。在这里，我们使用大鼠颈交感神经干(CST)，一种以非髓鞘干细胞为主要细胞类型的自主神经，通过逆转录-聚合酶链式反应分离锌指蛋白(ZFP)的cDNAs。我们分离到29个独特的ZFP序列，其中锌增殖1(Zipro1)是最丰富的。我们发现，在CST横断后，无髓鞘CST干细胞中Zipro1mRNA的表达水平增加了一倍，并且Zipro1蛋白的表达增加。我们还确定了Zipro1在TSCS中的表达，并且它的水平随着骨骼肌去神经支配而增加。因此，Zipro1是一个很好的候选转录因子，通常参与非髓鞘干细胞的激活，特别是TSCs。
Terminal Schwann cells (tSCs) are non-myelinating glia that wrap the nerve terminal at the neuromuscular junction. They are required for the maintenance of the neuromuscular synapse and are likely to play essential roles in the restoration of synaptic connections after nerve injury. tSCs acquire a reactive phenotype after nerve damage characterized by the extension of cellular processes that may facilitate reinnervation. The molecular signaling events underpinning the tSC reactive state remain elusive, in particular, little is known about transcription factors involved in the transcriptional reprogramming during tSC activation. Prior research implicated nine members of the zinc-finger transcription factor family in Schwann cell (SC) development and myelination, and levels of one such protein were reported increased in other non-myelinating SCs after denervation. We hypothesize that zinc-finger transcription factors could play a role during tSC activation. Because of their relative paucity, tSCs are difficult to study molecularly. Here, we used the rat cervical sympathetic trunk (CST), an autonomic nerve in which non-myelinating SCs are the predominant cell type, to isolate zinc-finger protein (ZFP) cDNAs by reverse transcriptase–polymerase chain reaction. We isolated 29 unique ZFP sequences of which zinc proliferation 1 (Zipro1) was the most abundant. We found that after CST transection, levels for Zipro1 mRNA doubled and that Zipro1 protein expression increased in non-myelinating CST SCs. We also determined that Zipro1 is expressed in tSCs and its levels increased following skeletal muscle denervation. Thus, Zipro1 is a good candidate for a transcription factor involved in activation of non-myelinating SCs in general, and tSCs in particular.