The structure of human 5-lipoxygenase.
The structure of human 5-lipoxygenase.
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DOI：
10.1126/science.1197203
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发表时间：
2011-01-14
期刊：
影响因子：
--
通讯作者：
Newcomer ME
中科院分区：
文献类型：
--
作者：
Gilbert NC;Bartlett SG;Waight MT;Neau DB;Boeglin WE;Brash AR;Newcomer ME
促炎症白三烯和抗炎脂蛋白的合成都需要5-脂氧合酶(5-LOX)。5-LOX的活性是短暂的，显然部分是由于酶的内在不稳定性。我们鉴定了一个5-LOX特异的不稳定序列，该序列参与定位与催化铁结合的羧基末端。在此，我们报道了通过替换该序列稳定的人5-LOX在2.4？分辨率下的晶体结构。
The synthesis of both pro-inflammatory leukotrienes and anti-inflammatory lipoxins requires the enzyme 5-lipoxygenase (5-LOX). 5-LOX activity is short-lived, apparently in part due to an intrinsic instability of the enzyme. We identified a 5-LOX-specific destabilizing sequence that is involved in orienting the carboxy-terminus which binds the catalytic iron. Herein we report the crystal structure at 2.4 Å resolution of human 5-LOX stabilized by replacement of this sequence.