Worldwide circulation of HSV-2 × HSV-1 recombinant strains.
Worldwide circulation of HSV-2 × HSV-1 recombinant strains.
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DOI：
10.1038/srep44084
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发表时间：
2017-03-13
影响因子：
4.6
通讯作者：
Johnston C
中科院分区：
文献类型：
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作者：
Koelle DM;Norberg P;Fitzgibbon MP;Russell RM;Greninger AL;Huang ML;Stensland L;Jing L;Magaret AS;Diem K;Selke S;Xie H;Celum C;Lingappa JR;Jerome KR;Wald A;Johnston C
智人拥有两种不同的、具有重要医学意义的单纯疱疹病毒，即单纯疱疹病毒(HSV)-1和HSV-2，据估计，它们在600-800万年前存在分歧。出乎意料的是，我们发现流通中的HSV-2毒株可以在三个不同的基因中包含HSV-1DNA片段。使用来自北美、南美和非洲的150多个生殖器拭子，我们在全球范围内检测到了重组体。一个 >；457Basepair(BP)单纯疱疹病毒1型 × 单纯疱疹病毒2型交叉，然后反向重组到单纯疱疹病毒2型，简单地解释了常见的、广泛分布的基因UL39型。单纯疱疹病毒1型基因UL29和UL30内的 >；244和 >；539 碱基片段已接近固定，少数毒株保留了我们推测为原始单纯疱疹病毒2型的序列。我们的数据增加了之前的体外和动物工作，表明在体内细胞与HSV-1和HSV-2的联合感染在自然人类宿主中产生了可行的跨物种重组体。
Homo sapiens harbor two distinct, medically significant species of simplexviruses, herpes simplex virus (HSV)-1 and HSV-2, with estimated divergence 6–8 million years ago (MYA). Unexpectedly, we found that circulating HSV-2 strains can contain HSV-1 DNA segments in three distinct genes. Using over 150 genital swabs from North and South America and Africa, we detected recombinants worldwide. Common, widely distributed gene UL39 genotypes are parsimoniously explained by an initial >457 basepair (bp) HSV-1 × HSV-2 crossover followed by back-recombination to HSV-2. Blocks of >244 and >539 bp of HSV-1 DNA within genes UL29 and UL30, respectively, have reached near fixation, with a minority of strains retaining sequences we posit as ancestral HSV-2. Our data add to previous in vitro and animal work, implying that in vivo cellular co-infection with HSV-1 and HSV-2 yields viable interspecies recombinants in the natural human host.