Gene profiling in Pap-cell smears of high-risk human papillomavirus-positive squamous cervical carcinoma
Gene profiling in Pap-cell smears of high-risk human papillomavirus-positive squamous cervical carcinoma
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DOI：
10.1016/j.ygyno.2006.12.032
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发表时间：
2007-05-01
影响因子：
4.7
通讯作者：
Czerwenka, Klaus
中科院分区：
文献类型：
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作者：
Manavi, Mahmood;Hudelist, Gernot;Czerwenka, Klaus
Objective.本研究旨在探讨宫颈拭子中良恶性鳞状细胞的差异表达基因和基因表达谱，以评估宫颈恶性肿瘤的生物学行为和临床结局。采用cDNA芯片技术对6例高危型人乳头瘤病毒（HR-HPV（+））宫颈癌患者和6例HPV阴性宫颈癌患者的宫颈鳞状细胞进行分析。多个基因如MET（c-met）、Nm 23-H1（NME 1）、EGFR、KGFR、Nm 23-H2（NME 2）、ERBB 2（c-erbB-2）、细胞周期蛋白依赖性激酶的cDNA过表达。抑制因子4（CDKN 2A，p16（INK 4A））、细胞角蛋白8（K-RT 8）、KRAS（K-ras）、FLT 1、KGF（FGF 7）、BCL 2样蛋白2（BCL 2L 2）、ERBB 4、MYCN（N-myc）、细胞周期蛋白D1（CCND 1）、KIT（c-kit）、分泌型磷蛋白1（SPP 1）和STAT 1在宫颈鳞状细胞癌（CSCC）中表达显著。CSCC中有13个基因表达下调，包括白细胞介素1 α（伊利亚）、转化生长因子受体β超家族（TGF β; TGFB）、胰岛素样生长因子结合蛋白（IGFBPs）和整合素家族（ITGA 6、ITGB 1）。本研究旨在探讨HR-HPV（-）和（+）宫颈鳞状细胞和CSCC的基因表达谱。我们观察了基因表达模式和信号通路，使研究人员能够区分良性鳞状宫颈细胞和CSCC与HPV感染。(c)2007年爱思唯尔公司All rights reserved.
Objective. The purpose of the study was to investigate benign and malignant squamous cervical cells obtained by cervical swabs with regard to differentially expressed genes and gene expression profiling, in order to evaluate the biological behavior and clinical outcome of cervical malignancies.Methods. Cervical squamous cells from six women with high-risk human papillomavirus positive [HR-HPV(+)] cervical carcinoma and from six HPV-negative women with normal ectocervical cells were analyzed by cDNA array.Results. cDNA over-expression of several genes such as MET (c-met), Nm23-H1 (NME1), EGFR, KGFR, Nm23-H2 (NME2), ERBB2 (c-erbB-2), cyclin-dependent kinase. inhibitor 4 (CDKN2A, p16(INK4A)), cytokeratin 8 (K-RT8), KRAS (K-ras), FLT1, KGF (FGF7), BCL2-like 2 protein (BCL2L2), ERBB4, MYCN (N-myc), cyclin D1 (CCND1), KIT (c-kit), secreted phosphoprotein 1 (SPP1) and STAT1, was significant in cervical squamous cell carcinoma (CSCC). Gene expression was downregulated for 13 genes in CSCC, such as interleukin 1 alpha (ILIA), the transforming growth factor receptor beta superfamily (TGF beta; TGFB), some members of the insulin-like growth factor binding proteins (IGFBPs) and the integrin family (ITGA6, ITGB1).Conclusion. This study was focused on the gene expression profiling of HR-HPV(-) and (+) cervical squamous cells and CSCC obtained by cytobrush. We observed gene expression patterns and signaling pathways that permit the investigator to distinguish between benign squamous cervical cells and CSCC with and without HPV infection. (c) 2007 Elsevier Inc. All rights reserved.