Antinociceptive effect of methyleugenol on formalin-induced hyperalgesia in mice
Antinociceptive effect of methyleugenol on formalin-induced hyperalgesia in mice
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DOI：
10.1016/j.ejphar.2006.09.020
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发表时间：
2006-12-28
影响因子：
5
通讯作者：
Miyamoto, Ken-ichi
中科院分区：
文献类型：
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作者：
Yano, Shingo;Suzuki, Yasuyuki;Miyamoto, Ken-ichi
采用小鼠福尔马林试验，研究了从亚洲细辛根中分离得到的精油甲基丁香酚的抗痫作用。口服10 mg/kg甲基丁香酚显著减少了第二阶段的舔和咬行为持续时间，而不影响第一阶段的行为，双氯芬酸（一种非甾体抗炎药）也是如此。甲基丁香酚还能抑制鞘内注射n-甲基- d -天冬氨酸（NMDA）诱导的疼痛相关行为，而双氯芬酸对这些行为没有影响。甲基丁香酚的这些作用被γ -氨基丁酸（GABA）拮抗剂双球茎碱（bicuculline）抑制。Muscimol是一种GABAA激动剂，在福尔马林测试和nmda诱导的行为方面表现出与甲基丁香酚相同的作用。甲基丁香酚不影响环氧化酶-1和-2活性。这些结果表明，甲基丁香酚对福尔马林诱导的第二阶段疼痛的抗痛觉作用可能是由于通过GABAA受体抑制NMDA受体介导的痛觉过敏。0 2006 Elsevier b.v.；版权所有。
The effects of methyleugenol, an essential oil isolated from Asiasari radix, on antinociception were examined using the formalin test in mice. Oral administration of 10 mg/kg methyleugenol significantly decreased the duration of licking and biting behavior in the second phase without affecting that of the first phase, as did diclofenac, a non-steroidal anti-inflammatory drug. Methyleugenol also inhibited pain-related behaviors induced by intrathecal injection of N-methyl-D-aspartic acid (NMDA), while diclofenac did not affect these behaviors. These effects of methyleugenol were suppressed by bicuculline, a gamma-aminobutyric acid(A) (GABA(A)) antagonist. Muscimol, a GABAA agonist, displays the same action as methyleugenol with respect to the formalin test and NMDA-induced behaviors. Methyleugenol did not affect cyclooxygenase-1 and -2 activities. These results suggest that the antinociceptive effect of methyleugenol on the second phase of formalin-induced pain may be due to the inhibition of NMDA receptor-mediated hyperalgesia via GABAA receptors. 0 2006 Elsevier B.V.. All rights reserved.