Circadian rhythms in murine pups develop in absence of a functional maternal circadian clock
Circadian rhythms in murine pups develop in absence of a functional maternal circadian clock
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DOI：
10.1177/0748730406286264
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发表时间：
2006-04-01
影响因子：
3.5
通讯作者：
Albrecht, U
中科院分区：
文献类型：
--
作者：
Jud, C;Albrecht, U
采用遗传方法来研究小鼠幼崽昼夜节律的出现是否依赖于功能正常的母体生物钟。携带 mPer1(Brdm1)/Per2(Brdm1) 或 mPer2(Brdm)/Cry1(-/-) 双突变基因型的心律失常雌性与野生型雄性在持续黑暗下杂交。杂合子后代具有功能性生物钟的遗传构成。心律失常的 mPer1(Brd1)/Per2(Brdm1) 和 mPer2(Brdm1)/Cry1(-/-) 母亲在没有外部时间的持续黑暗中所生的个体幼崽发展出正常的昼夜节律，但与野生型动物相比，它们的生物钟彼此不太同步。这些发现表明，昼夜节律的发育并不依赖于母体组织中的功能性昼夜节律时钟，扩展了之前从 SCN 受损母亲所生的幼崽中获得的发现。
A genetic approach was used to investigate whether the emergence of circadian rhythms in murine pups is dependent on a functional maternal clock. Arrhythmic females bearing either the mPer1(Brdm1)/Per2(Brdm1) or mPer2(Brdm)/Cry1(-/-) double-mutant genotype were crossed with wild-type males under constant darkness. The heterozygous offspring have the genetic constitution for a functional circadian clock. Individual pups born to arrhythmic mPer1(Brd1)/Per2(Brdm1) and mPer2(Brdm1)/Cry1(-/-) mothers in constant darkness without external zeitgeber developed normal circadian rhythms, but their clocks were less synchronized to each other compared to wild-type animals. These findings indicate that development of circadian rhythms does not depend on a functional circadian clock in maternal tissue, extending previous findings obtained from pups born to SCN-lesioned mothers.