Association of DNA primase with the beta/gamma subunits of DNA polymerase alpha from Drosophila melanogaster embryos.
Association of DNA primase with the beta/gamma subunits of DNA polymerase alpha from Drosophila melanogaster embryos.
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DOI：
10.1016/s0021-9258(17)44626-6
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发表时间：
1983-08
期刊：
影响因子：
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通讯作者：
L. Kaguni;J. Rossignol;R. Conaway;G. Banks;I. Lehman
中科院分区：
文献类型：
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作者：
L. Kaguni;J. Rossignol;R. Conaway;G. Banks;I. Lehman
果蝇早期胚胎完整DNA聚合酶α在天然甘油梯度共沉淀物中的DNA聚合酶和启动酶活性。然而，在含有2.8M尿素的甘油梯度中，酶的活性在3.4M尿素处理后被分离。DNA聚合酶活性所需的182,000个道尔顿α亚基(Kaguni，L.S.，Rossignol，J.-M.，Conaway，R.C.，和Lehman，I.R.(1983)Proc.娜塔莉。阿卡德。SCI。美国A 80,2221-2225)不是DNA底物酶活性所必需的。相反，启动酶活性存在于60,000道尔顿(贝塔)和/或50,000道尔顿(伽马)亚基中。没有发现与与完整酶共同纯化的73,000-道尔顿多肽有关的聚合酶或启动酶。
The DNA polymerase and primase activities of the intact DNA polymerase alpha from early embryos of Drosophila melanogaster co-sediment in native glycerol gradients. However, the activities are separated in glycerol gradients containing 2.8 M urea after treatment of the enzyme with 3.4 M urea. The 182,000-dalton alpha subunit which is required for DNA polymerase activity (Kaguni, L.S., Rossignol, J.-M., Conaway, R. C., and Lehman, I.R. (1983) Proc. Natl. Acad. Sci. U. S.A. 80, 2221-2225) is not required for DNA primase activity. Instead, primase activity resides in the 60,000-dalton (beta) and/or the 50,000-dalton (gamma) subunit. Neither polymerase nor primase has been found in association with the 73,000-dalton polypeptide which co-purifies with the intact enzyme.