PLASMA LACTIC DEHYDROGENASE ELEVATING AGENT OF MICE DISTRIBUTION IN TISSUES AND EFFECT ON LACTIC DEHYDROGENASE ISOZYME PATTERNS
PLASMA LACTIC DEHYDROGENASE ELEVATING AGENT OF MICE DISTRIBUTION IN TISSUES AND EFFECT ON LACTIC DEHYDROGENASE ISOZYME PATTERNS
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DOI：
10.1139/m63-008
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发表时间：
1963-01-01
影响因子：
2.8
通讯作者：
SWIM, HE
中科院分区：
文献类型：
--
作者：
PLAGEMANN, PGW;CHAN, KKW;SWIM, HE
已证实，携带许多可移植肿瘤的小鼠感染了病毒样因子，该因子导致无肿瘤的感染小鼠血浆乳酸脱氢酶活性升高5- 10倍。该制剂与多瘤病毒不同，对成年大鼠和仓鼠没有影响。在感染后36小时内观察到109至1010 ID 50/ml血浆的最大滴度。随后滴度降至106 - 107 ID 50/ml，此后保持恒定。血浆乳酸脱氢酶在感染后约96小时达到最大活性，并无限期地保持升高。该病毒以相对较高的浓度存在于粪便和各种组织和器官中。肝脏和脾脏产生最高滴度。从小鼠组织中分离出五种乳酸脱氢酶同工酶。小鼠感染导致血浆中最慢迁移的同工酶增加。发现肝脏、脾脏和红细胞均仅含有这种同工酶，而其他组织和器官则含有乳酸脱氢酶同工酶的混合物。携带肿瘤的小鼠的血浆中含有比没有肿瘤的感染小鼠更多的最慢迁移的同工酶。
It was confirmed that mice bearing many transplantable tumors are infected with a virus-like agent which causes a 5- to 10-fold elevation in the plasma lactic dehydrogenase activity of infected mice without tumors. The agent is non-identical with the polyoma virus and without effect on adult rats and hamsters. Maximum titers of 109 to 1010 ID50 per ml of plasma were observed within 36 hours after infection. Subsequently the titer decreased to 106 to 107 ID50 per ml and remained constant thereafter. The plasma lactic dehydrogenase reached maximum activity about 96 hours after infection and remained elevated indefinitely. The virus was present in feces and in a variety of tissues and organs in relatively high concentrations. Liver and spleen yielded the highest titers. Five electrophoretically distinct forms (isozymes) of lactic dehydrogenase were separated from mouse tissues. Infection of mice resulted in an increase of the slowest migrating isozyme in the plasma. Liver, spleen, and erythrocytes were each found to contain only this isozyme while other tissues and organs contained mixtures of lactic dehydrogenase isozymes. The plasma of tumor-bearing mice contained more of the slowest migrating isozyme than infected mice without tumors.