Prevotella bivia as a source of lipopolysaccharide in the vagina
Prevotella bivia as a source of lipopolysaccharide in the vagina
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DOI：
10.1016/j.anaerobe.2008.08.002
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发表时间：
2008-11-01
期刊：
影响因子：
2.3
通讯作者：
Faro, Sebastian
中科院分区：
文献类型：
--
作者：
Aroutcheva, Alla;Ling, Zaodung;Faro, Sebastian
目的：比较细菌性阴道病(BV)患者与非细菌性阴道病(BV)患者阴道分泌脂多糖(LPS)浓度，探讨内毒素与普雷沃特氏菌定植密度的相关性，以及内毒素对多巴胺神经元(DA)丢失的影响。方法：采用定量聚合酶链式反应检测43例BV患者和59例非BV患者阴道冲洗液中的细菌数量，用凝胶凝块法检测内毒素浓度。普氏杆菌、阴道加德纳氏菌和大肠杆菌的超声细胞提取物也被检测为产生内毒素。结果：有BV症状组大鼠嘴唇中位数浓度显著高于无症状组(分别为3235.0和46.4EU/ml，P<；0.001)，BV组大鼠双唇定植密度也明显高于无症状组(分别为0.06±0.36vs5.4+/2.2log(10)cfu/ml，P<；0.001)。普氏杆菌细胞裂解物的内毒素浓度(10,713.0+/-306.6 EU/ml)高于大肠埃希菌(4679.0+/-585.3 EU/ml)或阴道毛滴虫(0.07+/-0.01EU/ml)。用BV阴性患者阴道冲洗液培养的DA神经元损失率为20~27%，用BV阴性者阴道冲洗液处理的DA神经元损失率为58~97%。结论：P.bivia产生较高浓度的内毒素，可能是造成DA神经元损伤的有毒阴道环境。(C)2008爱思唯尔有限公司。保留所有权利。
Objectives: To compare vaginal lipopolysaccharides (LPS) concentrations between patients with and without bacterial vaginosis (BV), to evaluate the correlation between Prevotella bivia colonization density and LPS concentration, and to determine the impact of LPS on loss of dopamine neurons (DA).Methods: Vaginal washes obtained from patients with (n = 43) and without (n = 59) BV were tested for quantity of P. bivia cells using quantitative PCR and for concentrations of LPS using the Limulus Amebocyte Lysate gel clot method. Prevotella bivia, Gardnerella vaginalis and Escherichia coli sonicated cell extracts were also tested for LPS production. DA neuron cells obtained from embryonic day (E) 14.5 pregnant rats were exposed to fluid from eight vaginal washes; tyrosine hydrolase immunoreactive staining was applied for visualization and cell counts.Results: The median LIPS concentrations were dramatically higher among patients who had symptoms of BV compared to those who did not have symptoms (3235.0 vs 46.4 EU/ml, respectively, P < 0.001); patients who had BV also had much higher colonization densities of P. bivia (0.06 +/- 0.36 vs 5.4 +/- 2.2 log(10) CFU/ml, respectively, P < 0.001). Prevotella bivia cell lysates resulted in a higher LPS concentration (10,713.0 +/- 306.6 EU/ml) than either E coli (4679.0 +/- 585.3 EU/ml) or G. vaginalis (0.07 +/- 0.01 EU/ml of LPS). The loss of DA neuron was 20-27% in cultures treated with vaginal washes from BV-negative patients and 58-97% in cultures treated with vaginal washes from patients with BV.Conclusion: P. bivia produces high LPS concentration, which may create a toxic vaginal environment that damages DA neurons. (C) 2008 Elsevier Ltd. All rights reserved.