Compartments of sodium in a small artery.
Compartments of sodium in a small artery.
复制标题
小动脉中的钠室。
DOI：
10.1139/y68-100
复制
发表时间：
1968
影响因子：
2.1
通讯作者：
C. L. Friedman
中科院分区：
文献类型：
--
作者：
S. Friedman;B. Gustafson;D. Hamilton;C. L. Friedman
在改良的Krebs培养基中，活尾动脉样品在冷却过程中获得Na以换取K，并在再加热过程中逆转这一过程。该过程在实验误差范围内，通常以1:1的比例进行，并且具有较长的时间常数，在冷却后3小时内交换约8至9 meq/100 g干重，在24小时内可达15 meq/100 g。通过隔夜（18小时）冷却制备的组织再加热3小时，足以交换至少10 meq/100 g。在tris缓冲培养基中培养的组织中，钠的第二组分约为10 meq/100 g，显然是细胞旁的，因为它在暴露于无钠环境30分钟内完全动员，即使在2°。因为即使在这种2°的培养基中暴露3小时也不能调动代谢依赖的细胞Na，因此有可能区分这两种成分。也有证据表明，通常与钾积累有关的钠转运可以在没有钾积累的情况下发生。
Viable tail artery samples, in a modified Krebs medium, gain Na in exchange for K during cooling and reverse the process during rewarming. The process, within the margin of experimental error, is usually in a 1:1 ratio and has a long time constant, about 8 to 9 meq/100 g dry weight being exchanged within 3 h of cooling, and up to 15 meq/100 g within 24 h. Three hours of rewarming of tissues prepared by overnight (18 h) cooling is sufficient for an exchange of at least 10 meq/100 g. A second component of Na, equal to approximately 10 meq/100 g in tissue incubated in a Tris-buffered medium , is apparently paracellular, for it is fully mobilized within 30 min of exposure to a sodium-free environment, even at 2°. Since even 3 h of exposure to such a medium at 2° fails to mobilize metabolism-dependent cellular Na it is possible to differentiate these two components. Evidence is also presented to show that Na transport, ordinarily associated with K accumulation, can occur without it.