Experimental evaluation of myocardial preservation techniques: V. A. membrane-stabilizing agent, procaine hydrochloride.
Experimental evaluation of myocardial preservation techniques: V. A. membrane-stabilizing agent, procaine hydrochloride.
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心肌保存技术的实验评价：V.A.膜稳定剂、盐酸普鲁卡因。
DOI：
10.1016/s0003-4975(10)61274-x
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
A. Wakabayashi
中科院分区：
文献类型：
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作者：
T. Nishi;J. Guilmette;A. Wakabayashi
以离体兔心乳头肌收缩力为指标，观察盐酸普鲁卡因对缺氧心脏的保护作用。将54个实验分为9组，每组6个实验，其中缺氧时间（30、45、60分钟）和普鲁卡因浓度（溶液1或对照，无普鲁卡因;溶液2，0.03%;溶液3，0.3%）为变量。与溶液2或1相比，溶液3的心脏停搏显著更快（p <0.01）。缺氧30分钟后，溶液1的收缩性恢复率为81.1 ± 4.2%，溶液2为90.7 ± 3.4%，溶液3为90.6 ± 3.3%。缺氧45分钟后，对照组的恢复率为31.8 ± 19.0%，0.03%普鲁卡因组为67.5 ± 15.8%，0.3%普鲁卡因组为58.1 ± 22.8%。缺氧60分钟后，对照组的回收率为2.2 ± 2.7%，0.03%普鲁卡因组为18.7 ± 14.1%，0.3%普鲁卡因组为5.0 ± 7.3%。在每个缺氧期后，最好的恢复发生在那些被溶液2阻止的心脏中。虽然普鲁卡因组之间的恢复差异无统计学意义，但用溶液3停搏的心脏达到完全功能恢复所需的时间较长，本研究表明普鲁卡因本身对缺氧心脏具有保护作用，尽管高浓度（0.3%）的普鲁卡因使心脏迅速停搏，但它延迟了收缩的恢复。我们建议在心脏停搏液中使用0.03%普鲁卡因。
The protective effects of procaine hydrochloride on the anoxic heart were investigated using the papillary muscle contractility of an isolated, blood-perfused rabbit heart as an index of myocardial function. Fifty-four experiments were divided into nine groups of six experiments each, in which anoxic periods (30, 45, 60 minutes) and concentration of procaine (solution 1 or control, no procaine; solution 2, 0.03%; and solution 3, 0.3%) were the variables. The hearts arrested significantly faster with solution 3 compared with solution 2 or 1 (p< 0.01). After 30 minutes of anoxia, the recovery of the contractility was 81.1 ± 4.2% with solution 1, 90.7 ± 3.4% with solution 2, and 90.6 ± 3.3% with solution 3. After 45 minutes of anoxia, the recovery was 31.8 ± 19.0% with control, 67.5 ± 15.8% with 0.03% procaine, and 58.1 ± 22.8% with 0.3% procaine. After 60 minutes of anoxia, the recovery was 2.2 ± 2.7% with control, 18.7 ± 14.1% with 0.03% procaine, and 5.0 ± 7.3% with 0.3% procaine. After each anoxic period, the best recovery occurred with those hearts arrested by solution 2. Although the difference in recovery between the procaine groups was not statistically significant, a longer time was required for the hearts arrested with solution 3 to reach full functional recovery.This study indicated that procaine per se has a protective effect on the anoxic hearts and that although high concentration (0.3%) of procaine arrests the heart rapidly, it delays the recovery of contractions. We recommend the use of 0.03% procaine in cardioplegic solution.