Protoplast fusion between Lycopersicon esculentum and L. peruvianum-complex: somatic embryogenesis, plant regeneration and morphology
Protoplast fusion between Lycopersicon esculentum and L. peruvianum-complex: somatic embryogenesis, plant regeneration and morphology
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番茄和秘鲁番茄复合体之间的原生质体融合：体细胞胚胎发生、植物再生和形态
DOI：
10.1007/s002990050433
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发表时间：
1998
影响因子：
6.2
通讯作者：
T. Adachi
中科院分区：
文献类型：
--
作者：
L. Chen;T. Adachi
Somatic hybrids were obtained by polyethylene glycol fusion of cotyledon protoplasts ofLycopersicon esculentumMill. cv. Kyoryokutoko treated with iodoacetamide (IOA) and suspension-culture-derived protoplasts ofL.peruvianum(PI270435) orL.chilense(PI128652). The hybrids were selected by a multiple-step selection procedure relying on the different colors of the fusion partners, IOA treatment of cotyledon protoplasts, and the use of a culture medium which only allowed cotyledon protoplasts to regenerate. The somatic embryos were derived from greenish calli that formed from the fusion mixtures, developed progressively through the globular, heart, and torpedo stages, and finally formed complete plantlets. The excised torpedo-stage embryos could be propagated on a modified medium. The morphology of the somatic hybrids were intermediate to their donor partners, and chromosome observations indicated that the hybrids were tetraploid, hexaploid, and aneuploid.
番茄与陆地番茄耐低温体细胞杂种
DOI：
--
发表时间：
2002
期刊：
SABRAO Journal of Breedingand Genetics 34
影响因子：
--
作者：
Chen;L.Z.;K.Murai;M.Inoue;Y.Kaneko;T.Tsutsumi;T.Adachi.
通讯作者：
T.Adachi.