Detection and characterization of diarrheagenic Escherichia coli from young children in Hanoi, Vietnam
Detection and characterization of diarrheagenic Escherichia coli from young children in Hanoi, Vietnam
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DOI：
10.1128/jcm.43.2.755-760.2005
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发表时间：
2005-02-01
影响因子：
9.4
通讯作者：
Weintraub, A
中科院分区：
文献类型：
--
作者：
Nguyen, TV;Le Van, P;Weintraub, A
腹泻仍然是发展中国家婴儿和儿童发病和死亡的最常见原因之一。大肠杆菌是引起腹泻的病原体中的一种新兴病原体。建立了一种适用性强的检测不同种类大肠杆菌的技术。大肠杆菌很重要。我们采用多重PCR技术，将8对肠聚集性大肠杆菌特异性引物组合。大肠杆菌（EAEC）、肠侵袭性E. coli（EIEC）、肠出血性E.大肠杆菌、肠致病性E.大肠杆菌（EPEC）和产肠毒素E.大肠杆菌（ETEC）。这有助于鉴别五种不同类型的致痢E.大肠杆菌粪便样品中的单一反应的同时。大肠埃希氏菌的感染率为100%。大肠埃希菌感染率腹泻组为22.5%，对照组为12%。在587份5岁以下腹泻越南儿童粪便样本中，该技术鉴定出132株产肠杆菌。大肠杆菌菌株。其中包括68份（11.6%）EAEC样本、12份（2.0%）EIEC样本、39份（6.6%）EPEC样本和13份（2.2%）ETEC样本。在249名年龄匹配的对照中，30份样品为产肠杆菌阳性。杆菌分布为18份样本（7.2%）为EAEC，11份样本（4.4%）为EPEC，1份样本（0.4%）为ETEC。
Diarrhea continues to be one of the most common causes of morbidity and mortality among infants and children in developing countries. Escherichia coli is an emerging agent among pathogens that cause diarrhea. The development of a highly applicable technique for the detection of different categories of diarrheagenic E. coli is important. We have used multiplex PCR by combining eight primer pairs specific for enteroaggregative E. coli (EAEC), enteroinvasive E. coli (EIEC), enterohemorrhagic E. coli, enteropathogenic E. coli (EPEC), and enterotoxigenic E. coli (ETEC). This facilitates the identification of five different categories of diarrheagenic E. coli from stool samples in a single reaction simultaneously. The prevalences of diarrheagenic E. coli were 22.5 and 12% in the diarrhea group and the control group, respectively. Among 587 fecal samples from Vietnamese children under 5 years of age with diarrhea, this technique identified 132 diarrheagenic E. coli strains. This included 68 samples (11.6%) with EAEC, 12 samples (2.0%) with EIEC, 39 samples (6.6%) with EPEC, and 13 samples (2.2%) with ETEC. Among the 249 age-matched controls, 30 samples were positive for diarrheagenic E. coli. The distribution was 18 samples (7.2%) with EAEC, 11 samples (4.4%) with EPEC, and 1 sample (0.4%) with ETEC.