Intestinal microbes influence the survival, reproduction and protein profile of Trichinella spiralis in vitro
Intestinal microbes influence the survival, reproduction and protein profile of Trichinella spiralis in vitro
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DOI：
10.1016/j.ijpara.2015.08.007
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发表时间：
2016-01-01
影响因子：
4
通讯作者：
Zhang, Xi-chen
中科院分区：
文献类型：
--
作者：
Jiang, Hai-yan;Zhao, Na;Zhang, Xi-chen
肠道微生物和寄生虫之间的相互作用在宿主免疫系统的发育中发挥着重要作用。然而，肠道微生物对旋毛虫的影响尚不清楚。这项工作的目的是探索微生物诱导的旋毛虫体外存活和繁殖的改变。为了进一步鉴定参与线虫对微生物反应的蛋白质和基因，通过 iTRAQ 耦合 LCMS/MS 技术对螺旋线虫进行定量蛋白质组分析，并使用定量实时 PCR 测量 mRNA 表达的变化。结果表明，嗜酸乳杆菌，尤其是保加利亚乳杆菌，显着提高了线虫的存活率和繁殖率。肠沙门氏菌，尤其是大肠杆菌 0157:H7 (EHEC)，具有相反的作用。遗传反应主要由肠出血性大肠杆菌激活。共鉴定并定量了514种蛋白质，其中与碳水化合物代谢相关的蛋白质存在比例较高。这些发现表明，一些肠道细菌对人类有益或有害，此外它们可能对寄生虫也有类似的有益或有害影响。这可能是由于特定基因和蛋白质表达的调节。我们的研究为利用研究哺乳动物肠道微生物所产生的知识开发针对寄生虫感染的疗法奠定了基础。 (C) 2015 年澳大利亚寄生虫学协会。由 Elsevier Ltd 出版。保留所有权利。
The interactions between intestinal microbes and parasitic worms play an essential role in the development of the host immune system. However, the effects of gut microbes on Trichinella spiralis are unknown. The aim of this work was to explore microbe-induced alterations in the survival and reproduction of T. spiralis in vitro. To further identify the proteins and genes involved in the response of nematodes to microbes, quantitative proteomic analysis of T. spiralis was conducted by iTRAQ-coupled LCMS/MS technology and quantitative real-time-PCR was used to measure changes in mRNA expression. The results showed Lactobacillus acidophilus, and especially Lactobacillus bulgaricus, significantly enhanced the survival and reproductive rates of nematodes. Salmonella enterica, and especially Escherichia coli 0157:H7 (EHEC), had opposite effects. Genetic responses were activated mainly by EHEC. A total of 514 proteins were identified and quantified, and carbohydrate metabolism-related proteins existed in a higher proportion. These findings indicated that some gut bacteria are friendly or harmful to humans and in addition they may have similar beneficial or detrimental effects on parasites. This may be due to the regulation of expression of specific genes and proteins. Our studies provide a basis for developing therapies against parasitic infections from knowledge generated by studying the gut microbes of mammals. (C) 2015 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.