Expression of Th-17 and RORγt mRNA in Behçet's Disease.
Expression of Th-17 and RORγt mRNA in Behçet's Disease.
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DOI：
10.12659/msm.881720
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发表时间：
2011-04
期刊：
影响因子：
--
通讯作者：
Hamzaoui A
中科院分区：
文献类型：
--
作者：
Hamzaoui K;Bouali E;Ghorbel I;Khanfir M;Houman H;Hamzaoui A
目的：探讨白塞病(BD)患者血浆IL-17水平及Th1 7细胞转录因子RoRγt的表达在BD发病机制中的作用。采集73例BD患者(其中活动期45例)、20例系统性红斑狼疮(SLE)和12例多发性硬化(MS)患者的血样。对12例BD患者的活动期和缓解期进行了调查。采用酶联免疫吸附试验(EL ISA)检测血浆IL-17A水平。用实时定量逆转录聚合酶链式反应检测相关基因的表达。以人脐静脉内皮细胞(HUVECs)为对照，研究了活动期BD伴结节红斑样皮疹患者Th17细胞的功能。外周血单个核细胞中存在Th1 7细胞和rORγt。活动期BD、MS和SLE患者外周血中Th17细胞百分率和产生IL-17A的能力均升高。我们观察到活动期BD患者的IL-17A可诱导HUVECs表达黏附分子信使RNA。RoRγt证实Th1 7细胞可能参与了BD患者IL-17A的升高。我们的结果表明，IL-17参与了炎症性疾病和活动性BD患者的活动性促炎模式。
To investigate plasma IL-17 level and the expression of Th17 cell transcription factor RORγt in the pathogenesis of Behçet’s Disease (BD). Blood samples were collected from 73 patients with BD (45 patients were in active stage), 20 systemic lupus erythematosus (SLE) and 12 multiple sclerosis patients (MS). Twelve patients with BD were investigated both in their active and remission stages. Samples were processed to detect IL-17A level in plasma by enzyme-linked immunosorbent assay (ELISA). Related gene expression was assessed by real-time reverse transcription polymerase chain reaction. Function of Th17 cells in active BD patients with erythema nodosum (EN)-like eruption was studied in relation to human umbilical vein endothelial cells (HUVECs). We demonstrated the presence of Th17 cells and RORγt among the peripheral blood mononuclear cells (PBMC). The percentage of circulating Th17 cells and the ability to produce interleukin-17A (IL-17A) were increased in samples derived from patients with active BD, MS and SLE patients. We observed that IL-17A from patients with active BD could induce adhesion molecule messenger RNA expression in HUVECs. RORγt determined Th17 cell might be involved with increased IL-17A in BD. Our results indicate that IL-17 contributes to the active proinflammatory pattern that is characteristic of inflammatory diseases and patients with active BD.
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