A MORPHOLOGICAL ANALYSIS OF EARLY IMPLANTATION STAGES IN RAT
A MORPHOLOGICAL ANALYSIS OF EARLY IMPLANTATION STAGES IN RAT
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DOI：
10.1002/aja.1001200202
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发表时间：
1967-01-01
影响因子：
--
通讯作者：
SCHLAFKE, S
中科院分区：
文献类型：
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作者：
ENDERS, AC;SCHLAFKE, S
利用Holtzman大鼠已知的排卵和受精顺序、桥胺蓝反应和电镜技术，对着床的第一阶段进行了研究。当胚泡被子宫内膜夹住并因此占据固定位置时（第5天晚上），着床开始。在这个阶段，滋养层细胞与子宫上皮细胞紧密结合，在某些地方有相互交错的微绒毛，但成纤维细胞的蜕膜化才刚刚开始。在植入的第二阶段，脱落的成纤维细胞在腔上皮周围形成杯状。此时可见滋养层细胞的细胞膜与腔上皮细胞的细胞膜粘附的证据。直接围绕腔上皮的成纤维细胞层变成上皮样，导致腔上皮与其血管形成相对隔离。这个阶段在第6天下午发展得很好。到第7天早上，腔上皮已经从形成的外胎盘锥的区域消失到胚外滋养层的水平。胚囊侧面的滋养层细胞直接与腔上皮细胞的残留基底膜接触，并通过该结构和小的结缔组织裂缝与基质细胞分离。上皮的基质反应和粘附的滋养层细胞的细胞膜的上皮细胞的细胞膜方面的去除和吞噬上皮元素的相对隔离的重要性进行了讨论，并描述了许多在该过程中观察到的细胞学特征。
By making use of the known sequence of ovula-tion and fertilization in Holtzman rats, the Pontamine Blue reaction, and electron microscopy, the first stages in implantation were studied. Implantation is initiated when the blastocyst becomes clasped by the endometrium and hence assumes a fixed position (evening of day 5). At this stage, the trophoblast cells are in close association with the uterine epithelial cells, with interdigitating microvilli in places, but decidualization of the fibroblasts is just beginning. In the second stage of implantation the decidualized fibroblasts form a cup around the luminal epithelium. Evidence of adhesion of the cell membranes of trophoblast cells with the cell membranes of luminal epithelial cells can be seen at this time. The layer of fibroblasts immediately surrounding the luminal epithelium becomes epithelioid, resulting in a relative isolation of the luminal epithelium from its vascularization. This stage is well-developed by the afternoon of day 6. By the morning of day 7, the luminal epithelium has disappeared from the region of the forming ectoplacental cone down to the level of the abembryonic trophoblast. The trophoblast cells on the lateral aspect of the blasto-cyst are directly in contact with the residual basement membrane of the luminal epithelial cells, and are separated by this structure and a small connective tissue cleft from the stromal cells. The importance of the relative isolation of the epithelium by the stromal reaction and the adhesion of the cell membrane of the trophoblast cells to the cell membranes of epithelial cells with regard to removal and phagocytosis of epithelial elements are discussed, and many of the cytological features observed during the process are described.