SARS-CoV-2 Antibody Binding and Neutralization in Dried Blood Spot Eluates and Paired Plasma.
SARS-CoV-2 Antibody Binding and Neutralization in Dried Blood Spot Eluates and Paired Plasma.
复制标题
SARS-CoV-2抗体在干血斑洗脱液和配对血浆中的结合和中和。
DOI：
10.1128/spectrum.01298-21
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发表时间：
2021-10-31
影响因子：
3.7
通讯作者：
Overbaugh J
中科院分区：
文献类型：
--
作者：
Itell HL;Weight H;Fish CS;Logue JK;Franko N;Wolf CR;McCulloch DJ;Galloway J;Matsen FA 4th;Chu HY;Overbaugh J
对严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）特异性抗体的大规模评估对于了解人群血清阳性率、保护相关性和疫苗引发反应的持久性至关重要。大多数SARS-CoV-2研究表征血浆/血清中的抗体应答。虽然这些样本可靠且广泛使用，但它们带来了一些后勤限制，例如需要静脉穿刺收集以及运输和储存冷链。干血斑（DBS）克服了这些障碍，因为它们可以通过手指针刺自我收集，并在环境温度下邮寄和储存。在这里，我们通过比较来自SARS-CoV-2恢复期和接种疫苗个体的配对血浆和DBS之间的几种抗体应答来评估DBS用于SARS-CoV-2抗体测定的适用性。我们发现，DBS不仅反映了血浆抗体结合酶联免疫吸附试验（ELISA）和抗原表位使用噬菌体展示，但也产生了SARS-CoV-2中和滴度，与配对血浆高度相关。通过使测定适应高通量384孔格式，进一步简化了中和测量。本研究支持采用DBS进行多种SARS-CoV-2结合和中和试验。重要性从静脉血中分离的血浆和血清代表了用于评价感染或接种疫苗后SARS-CoV-2抗体应答的常规样本类型。然而，这些样本的收集是侵入性的，需要训练有素的人员和设备进行立即处理。一旦采集，血浆和血清必须在低温下储存和运输。为了确定新出现的SARS-CoV-2变体的风险以及对自然感染和疫苗接种的免疫反应的持久性，有必要测量世界各地人群（包括资源有限地区）的各种抗体特征。因此，需要一种与这些环境兼容并适用于各种SARS-CoV-2抗体检测的采样方法，以继续了解和遏制COVID-19大流行。
Wide-scale assessment of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific antibodies is critical to understanding population seroprevalence, correlates of protection, and the longevity of vaccine-elicited responses. Most SARS-CoV-2 studies characterize antibody responses in plasma/sera. While reliable and broadly used, these samples pose several logistical restrictions, such as requiring venipuncture for collection and a cold chain for transportation and storage. Dried blood spots (DBS) overcome these barriers as they can be self-collected by fingerstick and mailed and stored at ambient temperature. Here, we evaluate the suitability of DBS for SARS-CoV-2 antibody assays by comparing several antibody responses between paired plasma and DBS from SARS-CoV-2 convalescent and vaccinated individuals. We found that DBS not only reflected plasma antibody binding by enzyme-linked immunosorbent assay (ELISA) and epitope profiles using phage display, but also yielded SARS-CoV-2 neutralization titers that highly correlated with paired plasma. Neutralization measurement was further streamlined by adapting assays to a high-throughput 384-well format. This study supports the adoption of DBS for numerous SARS-CoV-2 binding and neutralization assays. IMPORTANCE Plasma and sera isolated from venous blood represent conventional sample types used for the evaluation of SARS-CoV-2 antibody responses after infection or vaccination. However, collection of these samples is invasive and requires trained personnel and equipment for immediate processing. Once collected, plasma and sera must be stored and shipped at cold temperatures. To define the risk of emerging SARS-CoV-2 variants and the longevity of immune responses to natural infection and vaccination, it will be necessary to measure various antibody features in populations around the world, including in resource-limited areas. A sampling method that is compatible with these settings and is suitable for a variety of SARS-CoV-2 antibody assays is therefore needed to continue to understand and curb the COVID-19 pandemic.