Delayed‐type hypersensitivity induced by antigen pulsed, bone marrow‐derived macrophages
Delayed‐type hypersensitivity induced by antigen pulsed, bone marrow‐derived macrophages
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由抗原脉冲的骨髓源性巨噬细胞诱导的迟发型超敏反应
DOI：
10.1002/eji.1830100302
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发表时间：
1980
影响因子：
5.4
通讯作者：
Jacques F. Miller
中科院分区：
文献类型：
--
作者：
P. Mottram;Jacques F. Miller
在特定条件培养基中培养的小鼠骨髓细胞在7天后被发现几乎是纯的巨噬细胞。当用抗原脉冲时，这些细胞诱导并引发迟发型超敏反应（DTH），其效率和主要组织相容性复合物限制与抗原脉冲的腹膜渗出液细胞相同。通过补体介导的细胞毒性测定，这两种巨噬细胞群均具有20%的Ia+细胞。当去除大部分Ia+细胞时，剩余的抗原脉冲巨噬细胞不能在致敏小鼠中引起DTH。
Mouse bone marrow cells, cultured in specifically conditioned medium, were found to be virtually pure macrophages after 7 days. When pulsed with antigen, these cells induced and elicited delayed‐type hypersensitivity (DTH) with the same efficiency and major histocompatibility complex restrictions as antigen‐pulsed peritoneal exudate cells. Both of these macrophage populations had 20% Ia+ cells, as measured by complement‐mediated cytotoxicity. When most of the Ia+ cells were removed, the remaining antigen‐pulsed macrophages could not elicit DTH in sensitized mice.