A sandwich electrochemical immunosensor based on MXene@dual MOFs for detection of tumor marker CA125
A sandwich electrochemical immunosensor based on MXene@dual MOFs for detection of tumor marker CA125
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DOI：
10.1007/s00604-023-05719-w
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发表时间：
2023-03
影响因子：
5.7
通讯作者：
Lingli Qu;Mengdie Wu;Luyao Zhao;Jiang Li;Hongzhi Pan
中科院分区：
文献类型：
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作者：
Lingli Qu;Mengdie Wu;Luyao Zhao;Jiang Li;Hongzhi Pan
通过双金属-有机骨架（MOF）夹心策略，可以定量放大糖抗原125（CA 125）的检测信号。我们提出了一种通用的方法来合成均匀的MXene和MIL-101（Fe）-NH 2复合材料，该复合材料联合收割机结合了两种材料的优点，以构建具有优异性能的基层。MXene具有优异的导电性和高表面积。介孔MIL-101（Fe）-NH 2不仅提高了一抗的负载量，而且具有金属中心（Fe）的催化活性。制备了负载亚甲基蓝（MB）的UiO 66作为电化学免疫传感器信号标签，以实现对CA 125的检测。以MXene和MIL-101（Fe）-NH 2的混合物为底物，用富含氨基的壳聚糖固定。作为信号放大部分，UiO66@MB增强了二抗负载能力，并产生氧化还原信号，从而能够检测抗原物质。该电化学免疫传感器具有很高的灵敏度，检测限（LOD）为0.006 U/mL。因此，基于双MOF的免疫传感器为CA 125的早期诊断提供了一种新的方法。
The detection signal of carbohydrate antigen 125 (CA125) can be quantitatively amplified via the dual metal–organic framework (MOF) sandwich strategy. We propose a versatile method for synthesizing uniform MXene and MIL-101(Fe)-NH2composites that combine the advantages of both materials to build a base layer with superb performance. MXene exhibits excellent electrical conductivity and high surface area. The mesoporous MIL-101(Fe)-NH2not only increases the loading capacity of the primary antibody but also possesses the catalytic activity of the metal center (Fe). UiO66 loaded with methylene blue (MB) was fabricated as an electrochemical immunosensor signal tag to enable the detection of CA125. The mixture of MXene and MIL-101(Fe)-NH2prepared as the substrate was fixed by chitosan rich in amino groups. As the signal amplification sector, UiO66@MB enhanced secondary antibody loading capacity and generated a redox signal enabling the detection of antigenic substances. The proposed electrochemical immunosensor demonstrated high sensitivity with a low limit of detection (LOD) of 0.006 U/mL. Therefore, the dual MOF sandwich-based immunosensor provides a novel method for the early diagnosis of CA125.Graphical Abstract