Fabrication of three-dimensional scaffolds for heterogeneous tissue engineering
Fabrication of three-dimensional scaffolds for heterogeneous tissue engineering
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DOI：
10.1007/s10544-010-9425-2
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发表时间：
2010-08-01
影响因子：
2.8
通讯作者：
Chen, Shaochen
中科院分区：
文献类型：
--
作者：
Han, Li-Hsin;Suri, Shalu;Chen, Shaochen
生物医学支架的发展模拟异质细胞微环境对细胞命运的特定调节是非常有前途的组织工程。在本研究中，采用DMD-PP装置制备了具有异质结构的三维支架。在制造过程中，该装置可以有效地切换单体，形成具有局部不同材料性质的微观结构；材料性质排列的分辨率可与活体器官中功能亚基的特征尺寸相媲美，即100微米。这种DMD-PP装置的有效性通过具有异质荧光的木堆微观结构和具有选定蛋白质粘附位点的微孔细胞培养支架来证明。用微孔支架进行细胞培养实验，观察细胞选择性粘附。
The development of biomedical scaffolds mimicking a heterogeneous cellular microenvironment for a specified regulation of cell-fates is very promising for tissue engineering. In this study, three-dimensional scaffolds with heterogeneous microstructure were developed using a DMD-PP apparatus. During the fabrication process, this apparatus can efficiently switch monomers to form microstructures with localized, different material properties; the resolution in the arrangement of material properties is comparable to the characteristic size of functional subunits in living organs, namely, a hundred microns. The effectiveness of this DMD-PP apparatus is demonstrated by a woodpile microstructure with heterogeneous fluorescence and also by a microporous cell-culturing scaffold with selected sites for protein adhesion. Cell-cultivation experiment was performed with the microporous scaffold, in which selective cell adhesion was observed.