SOMATOTOPIC ORGANIZATION OF THE DORSAL COLUMN NUCLEI IN THE RAT - TRANSGANGLIONIC LABELING WITH B-HRP AND WGA-HRP
SOMATOTOPIC ORGANIZATION OF THE DORSAL COLUMN NUCLEI IN THE RAT - TRANSGANGLIONIC LABELING WITH B-HRP AND WGA-HRP
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DOI：
10.1016/0006-8993(91)91351-z
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发表时间：
1991-11-08
期刊：
影响因子：
2.9
通讯作者：
EGGER, MD
中科院分区：
文献类型：
--
作者：
MASLANY, S;CROCKETT, DP;EGGER, MD
为了分析皮肤初级传入纤维投射到大鼠背柱核的模式，将辣根过氧化物酶（HRP）为基础的示踪剂注射到前肢和后肢的一些离散区域。注射HRP后3 -4天，对大鼠进行经心灌注;切下60 μ m横截面，使用四甲基联苯胺法与HRP反应。用类霍乱毒素结合辣根过氧化物酶（B-HRP）或小麦胚芽凝集素结合辣根过氧化物酶（WGA-HRP）注射后的预测进行了比较。B-HRP和WGA-HRP产生类似的标记模式，但B-HR-P产生更大的标记强度和略大的投影面积。在楔状核（CN），HRP标记的初级传入小，划定的区域，如，从一个手指的皮肤的一部分，似乎是精确地限制在rostrocaudally定向列，很少或没有重叠（在内侧和背腹平面）到相邻区域。关于rostrocaudal组织，一个地区在CN含有密集的人口的皮肤初级传入纤维似乎是类似的中间，或集群，在猫和浣熊和部分圆形的灵长类动物的区域。投射模式非常一致，从大鼠到大鼠，但他们的躯体组织不同的电生理学研究所建议的：皮肤传入前肢数字1预测的CN的腹侧边缘附近，从数字5预测背内侧的数字1，从其余的数字的预测数字1和5之间的预测形成了一个新月。在薄束核，组织的皮肤传入投射从后肢数字是更复杂的变量比CN。
To analyze the patterns of cutaneous primary afferent fibers projecting to the dorsal column nuclei in the rat, horseradish peroxidase (HRP)-based tracers were injected intracutaneously into a number of discrete regions of the forelimbs and hindlimbs. Three-4 days following the HRP injections, the rats were perfused transcardially; 60-mu-m transverse sections were cut, and the HRP was reacted using the tetramethyl benzidine method. Comparisons were made of projections following injections with choleragenoid-conjugated horseradish peroxidase (B-HRP) or with wheat-germ agglutinin conjugated to horseradish peroxidase (WGA-HRP). B-HRP and WGA-HRP produced similar patterns of labelling, but B-HR-P produced greater intensity of labelling and slightly larger projection areas. In the cuneate nucleus (CN), HRP labelling of primary afferents from small, delimited regions, e.g., from a portion of the skin of a single digit, appeared to be precisely restricted in rostrocaudally oriented columns, with little or no overlap (in the mediolateral and dorsoventral plane) into adjacent regions. With respect to rostrocaudal organization, a region in the CN containing a dense population of cutaneous primary afferent fibers appeared to be similar to the middle, or cluster, region in cats and in raccoons and the pars rotunda in primates. Projection patterns were very consistent from rat to rat, but their somatotopic organization differed from that suggested by electrophysiological studies: cutaneous afferents from forelimb digit 1 projected near the ventral border of the CN; those from digit 5 projected dorsomedially to those from digit 1; the projections from the remaining digits formed a crescent between the projections from digits 1 and 5. In the gracile nucleus, the organization of cutaneous afferent projections from hindlimb digits was more variable and complex than that found in the CN.