Single-nucleotide-polymorphism typing and genetic relationships of Salmonella enterica serovar typhi isolates
Single-nucleotide-polymorphism typing and genetic relationships of Salmonella enterica serovar typhi isolates
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DOI：
10.1128/jcm.00720-07
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发表时间：
2007-11-01
影响因子：
9.4
通讯作者：
Lan, Ruiting
中科院分区：
文献类型：
--
作者：
Octavia, Sophie;Lan, Ruiting
伤寒沙门氏菌血清型伤寒杆菌是一个变异程度较低的克隆。我们开发了一种针对血清型伤寒杆菌的分子分型方法，利用38个全基因组单核苷酸多态性（SNP）作为通过聚合酶链反应 - 限制性内切酶消化检测的标记。所研究的73株全球血清型伤寒杆菌分离株被分为23种SNP图谱和4个不同的基因群。表达独特鞭毛抗原z66的血清型伤寒杆菌分离株被发现聚集在一起，并从祖先群分支出来，这表明血清型伤寒杆菌最初是单相的，只有H抗原，随后获得了z66抗原。使用38个SNP进行分型的鉴别力为0.87，并且至少16个SNP可用于实现相同水平的区分。我们开发的SNP分型方法将成为血清型伤寒杆菌全球流行病学研究的一个有价值的工具。
Salmonella enterica serovar Typhi is a clone with a low level of variation. We developed a molecular typing method for serovar Typhi using 38 genome-wide single-nucleotide polymorphisms (SNPs) as markers detected by PCR-restriction enzyme digestion. The 73 worldwide serovar Typhi isolates studied were separated into 23 SNP profiles and four distinct genetic groups. Serovar Typhi isolates expressing the unique flagellar antigen z66 were found to cluster together and branch off from the ancestral group, suggesting that serovar Typhi was initially monophasic with only an HI antigen and subsequently gained the z66 antigen. Typing using the 38 SNPs gave a discriminatory power of 0.87, and a minimum of 16 SNPs may be used to achieve the same level of differentiation. The SNP typing method we developed will be a valuable tool for global epidemiology studies of serovar Typhi.