Black tea increased survival of Caenorhabditis elegans under stress.
Black tea increased survival of Caenorhabditis elegans under stress.
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DOI：
10.1021/jf503120j
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发表时间：
2014-11
影响因子：
6.1
通讯作者：
Ligui Xiong;Jian-an Huang;Juan Li;Peng Yu;Zherui Xiong;Jian-wei Zhang;Yushun Gong;Zhong-Hua Liu-Zhong
中科院分区：
文献类型：
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作者：
Ligui Xiong;Jian-an Huang;Juan Li;Peng Yu;Zherui Xiong;Jian-wei Zhang;Yushun Gong;Zhong-Hua Liu-Zhong
本研究探讨了红茶（茶树）提取物（BTE）在秀丽隐杆线虫在各种非生物胁迫下的影响。结果表明，BTE提高线虫对渗透，热，紫外线照射处理的抗性。然而，BTE不能增加线虫的寿命在正常的培养条件下和MnCl 2诱导的毒性在我们使用的浓度。进一步的研究表明，BTE降低了活性氧，上调了一些抗氧化酶，包括GSH-PX，以及基因，如GSH-PX和SOD-3。然而，在mev-1突变体中仅观察到平均寿命的轻微延长，但无显著性。这些结果表明，BTE的抗氧化活性可能是必要的，但不足以防止老化到C。优雅此外，BTE增加应激反应基因，如sir-2.1和sek-1的mRNA水平。我们的发现表明BTE可能以sir.2.1依赖的方式增加耐热性和抗紫外线胁迫性。综合来看，BTE通过多种机制增强了C.优雅
The present study examined the effects of black tea (Camellia sinensis) extracts (BTE) in Caenorhabditis elegans under various abiotic stressors. Results showed BTE increased nematode resistance to osmosis, heat, and UV irradiation treatments. However, BTE could not increase nematodes' lifespan under normal culture conditions and MnCl2-induced toxicity at concentrations we used. Further studies showed that BTE decreased reactive oxygen species and up-regulated some antioxidant enzymes, including GSH-PX, and genes, such as gsh-px and sod-3. However, only a slight extension in mev-1 mutants mean lifespan was observed without significance. These results indicated that the antioxidant activity of BTE might be necessary but not sufficient to protect against aging to C. elegans. Moreover, BTE increased the mRNA level of stress-response genes such as sir-2.1 and sek-1. Our finding demonstrated BTE might increase heat and UV stress resistance in a sir.2.1-dependent manner. Taken together, BTE enhanced stress resistance with multiple mechanisms in C. elegans.