Studies on Indian Green Leafy Vegetables for Their Antioxidant Activity
Studies on Indian Green Leafy Vegetables for Their Antioxidant Activity
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DOI：
10.1007/s11130-008-0096-6
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发表时间：
2009-03-01
影响因子：
4
通讯作者：
Prakash, Jamuna
中科院分区：
文献类型：
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作者：
Gupta, Sheetal;Prakash, Jamuna
为了确定绿叶蔬菜(GLV)作为抗氧化剂的潜力，研究了苋菜、积雪草、九里香和胡芦巴的乙醇提取物在不同体系中不同浓度下的抗氧化活性。用磷钼法测定总抗氧化活性、1，1-二苯基-2-苦皮酰肼(DPPH)清除自由基活性、还原能力和亚铁离子螯合活性。分析GLV中抗坏血酸、总胡萝卜素、β-胡萝卜素和总多酚的含量。抗坏血酸、总胡萝卜素、β-胡萝卜素和总酚含量(单宁酸当量)分别为15.18~101.36、34.78~64.51、4.23~8.84和150.0~387.50 mg/100gGLV。在被测试的系统中，这些提取物被发现具有显著不同的抗氧化活性。总抗氧化活性以九里香最高(2,691.78 mU/g样品)，积雪草最低(623.78 mU/g样品)。测定了对DPPH有50%抑制作用的提取物浓度(IC(50))
To identify the potential of green leafy vegetables (GLV) as antioxidants, methanolic extracts of Amaranthus sp., Centella asiatica, Murraya koenigii and Trigonella foenum graecum were studied for their antioxidant activity in different systems at multiple concentrations. Total antioxidant activity assessed by phosphomolybdenum method, free radical scavenging activity by 1,1-diphenly-2-picryl hydrazyl (DPPH), reducing power and ferrous ion chelating activity were determined. The GLV were analyzed for ascorbic acid, total and beta-carotene and total polyphenol contents. The ascorbic acid, total carotene, beta-carotene and total phenolic content (tannic acid equivalents) of the GLV ranged between 15.18-101.36, 34.78-64.51, 4.23-8.84 and 150.0-387.50 mg/100 g GLV, respectively. The extracts were found to have significantly different levels of antioxidant activities in the systems tested. The total antioxidant activity was highest in Murraya koenigii (2,691.78 mu mol of ascorbic acid/g sample) and least in Centella asiatica (623.78 mu mol of ascorbic acid/g sample). The extract concentration causing 50% inhibition of DPPH (IC(50)) was determined (M. koenigii