The RING finger E3 ligase STRF1 is involved in membrane trafficking and modulates salt-stress response in Arabidopsis thaliana
The RING finger E3 ligase STRF1 is involved in membrane trafficking and modulates salt-stress response in Arabidopsis thaliana
复制标题
RING Finger E3 连接酶 STRF1 参与拟南芥的膜运输并调节盐胁迫反应。
DOI：
10.1111/tpj.12797
复制
发表时间：
2015-04-01
期刊：
影响因子：
7.2
通讯作者：
Xie, Qi
中科院分区：
文献类型：
--
作者：
Tian, Miaomiao;Lou, Lijuan;Xie, Qi
盐胁迫是植物生长发育的不利因素。对盐胁迫的反应已被证明涉及细胞内运输系统中的组分，以及泛素-蛋白酶体系统（UPS）的组分。在本文中，我们在拟南芥中发现了一个很少报道的参与盐胁迫反应的泛素连接酶，我们将其命名为STRF 1（Salt Tolerance Ring Finger 1）。STRF 1是RING-H2指蛋白的成员，我们证明它在体外具有泛素连接酶活性。我们还表明，STRF 1主要定位在质膜和细胞内的内体。strf 1 -1功能丧失突变体幼苗在根中表现出加速的内吞作用，并且改变了参与膜运输系统的几个基因的表达。此外，蛋白质运输抑制剂，布雷菲德菌素A（BFA），治疗增加了BFA机构在strf 1 -1突变体。该突变体还表现出对盐、离子和渗透胁迫的耐受性增加，盐胁迫期间活性氧的积累减少，AtRbohD的表达增加，AtRbohD编码参与H2 O2产生的烟酰胺腺嘌呤二核苷酸磷酸（NADPH）氧化酶。我们的结论是，STRF 1是一个膜运输相关的泛素连接酶，它有助于植物响应盐胁迫，通过监测细胞内膜运输和活性氧（ROS）的生产。
Salt stress is a detrimental factor for plant growth and development. The response to salt stress has been shown to involve components in the intracellular trafficking system, as well as components of the ubiquitin-proteasome system (UPS). In this article, we have identified in Arabidopsis thaliana a little reported ubiquitin ligase involved in salt-stress response, which we named STRF1 (Salt Tolerance RING Finger 1). STRF1 is a member of RING-H2 finger proteins and we demonstrate that it has ubiquitin ligase activity in vitro. We also show that STRF1 localizes mainly at the plasma membrane and at the intracellular endosomes. strf1-1 loss-of-function mutant seedlings exhibit accelerated endocytosis in roots, and have altered expression of several genes involved in the membrane trafficking system. Moreover, protein trafficking inhibitor, brefeldin A (BFA), treatment has increased BFA bodies in strf1-1 mutant. This mutant also showed increased tolerance to salt, ionic and osmotic stresses, reduced accumulation of reactive oxygen species during salt stress, and increased expression of AtRbohD, which encodes a nicotinamide adenine dinucleotide phosphate (NADPH) oxidase involved in H2 O2 production. We conclude that STRF1 is a membrane trafficking-related ubiquitin ligase, which helps the plant to respond to salt stress by monitoring intracellular membrane trafficking and reactive oxygen species (ROS) production.