Rat olfactory bulb and epithelium UDP-glucuronosyltransferase 2A1 (UGT2A1) expression: in situ mRNA localization and quantitative analysis
Rat olfactory bulb and epithelium UDP-glucuronosyltransferase 2A1 (UGT2A1) expression: in situ mRNA localization and quantitative analysis
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DOI：
10.1016/s0169-328x(01)00080-8
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发表时间：
2001-05-20
期刊：
影响因子：
--
通讯作者：
Goudonnet, H
中科院分区：
文献类型：
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作者：
Heydel, JM;Leclerc, S;Goudonnet, H
UDP-葡萄糖醛酸基转移酶(UGT)是一个多基因的酶家族，参与了多种内源和外源化合物的生物转化和消除。除了肝脏和其他组织中存在的各种UGT亚型外，UGT2A1亚型，也被称为嗅觉UGT，最初被认为只在鼻黏膜上皮细胞中表达。在这项工作中，我们利用原位杂交和定量逆转录聚合酶链式反应(RT-PCR)技术证明了UGT2A1在嗅球中的表达。在上皮内，UGT2A1mRNA主要见于支持细胞，在鲍曼腺细胞中表达较少。此外，原位杂交显示UGT2A1mRNA在嗅觉神经元核团中有表达。UGT2A1基因在嗅球内的神经元定位主要见于更深层的颗粒细胞。多标准定量RT-PCR方法的建立首先需要通过快速扩增cDNAEnds(RACE)-PCR来鉴定小鼠UGt2A1的cDNA.在大鼠和小鼠中，嗅球中的UGT2A1 mRNA水平似乎比上皮中的水平低6倍。UGT2A1在直接连接鼻部上皮和大脑的嗅球中的表达，强调了这种酶在保护大脑免受空气中危险化学物质伤害方面的潜在作用。(C)2001年爱思唯尔科学公司。版权所有。
UDP-glucuronosyltransferases (UGTs) form a multigenic family of enzymes involved in the biotransformation and elimination of numerous endo- and xenobiotic compounds. Beside the diverse UGT isoforms present in the liver as well as in other tissues, the UGT2A1 isoform, also called olfactory UGT, was initially thought to be expressed in the nasal epithelium only. In this work, we demonstrate the UGT2A1 mRNA expression in the olfactory bulb, using in situ hybridization and quantitative reverse transcription-polymerase chain reaction (RT-PCR) techniques. Within the epithelium, UGT2A1 mRNA is mainly found in the sustentacular cells and to a lesser extent in Bowman's gland cells. Moreover, in situ hybrization staining reveals UGT2A1 mRNA expression in the olfactory sensory neuron nuclei. Neuronal localization of UGT2A1 mRNA within the olfactory bulb is mainly found in the deeper granular cells. The development of the quantitative multistandard RT-PCR method firstly required characterization of the mouse Ugt2A1 cDNA by rapid amplification of cDNA ends (RACE)-PCR. UGT2A1 mRNA levels appear quantitatively six-fold lower in the olfactory bulb than in the epithelium, in both the rat and mouse. The expression of UGT2A1 in the olfactory bulb, which directly connects the nasal epithelium to the brain, emphasizes the potential role of this enzyme in the protection of the brain against airborne hazardous chemicals. (C) 2001 Elsevier Science BN. All rights reserved.