Topological properties and spatial organization of villous capillaries in normal and diabetic placentas
Topological properties and spatial organization of villous capillaries in normal and diabetic placentas
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DOI：
10.1159/000063692
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发表时间：
2002-05-01
影响因子：
1.7
通讯作者：
Karen, P
中科院分区：
文献类型：
--
作者：
Jirkovská, M;Kubínová, L;Karen, P
对9例正常胎盘和11例糖尿病胎盘的终绒毛毛细血管床的空间排列和复杂性进行了分析。标本采用系统随机取样，固定染色，石蜡包埋。从120毫米厚的标本切片中系统取样15个视场，并使用共聚焦激光扫描显微镜进行检查。利用共聚焦显微镜记录末端绒毛的一系列薄光学切片及其发育形态，作为绒毛毛细血管空间排列三维可视化的初始数据。通过聚焦绒毛，绘制绒毛毛细血管床示意图，统计冗余毛细血管连接，研究血管的拓扑结构和分支。研究发现，在正常胎盘和糖尿病胎盘中，绒毛毛细血管的基本排列是相似的。然而，在正常胎盘中，没有冗余连接的简单形式毛细血管床的比例明显更高，而在糖尿病胎盘中，每个绒毛的平均冗余连接数明显更高。综上所述，妊娠后期胎盘毛细血管的纵向生长和分支都导致了胎盘毛细血管床的增加，糖尿病绒毛的毛细血管分支更加强烈，毛细血管床更加复杂。版权所有(C) 2002 S. Karger AG，巴塞尔。
Spatial arrangement and complexity of the capillary bed of placental terminal villi were analyzed in 9 normal and 11 diabetic placentas. Specimens were taken by systematic random sampling, fixed and stained in toto, and embedded in paraffin. Fifteen fields of view were sampled systematically from 120-mum-thick sections of specimens and examined using a confocal laser scanning microscope. Series of thin optical sections of terminal villi and their developmental forms were recorded by the confocal microscope and used as initial data for three-dimensional visualization of the spatial arrangement of villous capillaries. Vascular topology and branching were studied by focusing through the villus, making a schematic drawing of the villous capillary bed and counting redundant capillary connections. It was found that the basic arrangement of villous capillaries is similar in both normal and diabetic placentas. Nevertheless, the proportion of simple forms of the capillary bed without redundant connections is significantly higher in normal placentas and the mean number of redundant connections per villus is significantly higher in diabetic placentas. It is concluded that both the longitudinal growth and branching of capillaries contribute to the increase in the placental capillary bed in late gestation and that the capillary bed of diabetic villi is more complicated due to more intense capillary branching. Copyright (C) 2002 S. Karger AG, Basel.