QTL mapping of grain weight in rice and the validation of the QTL qTGW3.2

QTL mapping of grain weight in rice and the validation of the QTL qTGW3.2
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水稻粒重QTL定位及QTL qTGW3.2验证

DOI:
10.1016/j.gene.2013.05.063
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发表时间:
2013-09-15
期刊:
影响因子:
3.5
通讯作者:
Pei-song, Hu
Pei-song, Hu
中科院分区:
生物学3区
文献类型:
--
作者:
Shao-qing, Tang;Gao-neng, Shao;Pei-song, Hu

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一个重组自交系(RIL)群体从一个爪哇类型(cv. D50)和indica type(cv.以HB 277为材料,对7个影响水稻千粒重的数量性状基因座(QTL)进行定位。这些位点分布在第2、3、5、6、8和10号染色体上。第3染色体QTL qTGW3.2在两年中稳定表达,并贡献9-10%的表型方差。从RIL群体中选择剩余杂合系(RHL),并使用其自交后代精细定位qTGW3.2。在这个“F-2”群体中,该QTL解释了约23%的方差,在随后的“F-2:3”世代中上升至近33%。qTGW3.2的物理位置被限制在一个类似的556 kb的区域,两侧是微卫星位点RM 16162和RM 16194。该区域还包含影响某些产量相关性状的其他因素,尽管qTGW3.2也可能以多效性方式影响这些性状。皇冠版权所有(C)2013由爱思唯尔B. V.出版保留所有权利。
A recombinant inbred line (RIL) population bred from a cross between a javanica type (cv. D50) and an indica type (cv. HB277) rice was used to map seven quantitative trait loci (QTLs) for thousand grain weight (TGW). The loci were distributed on chromosomes 2, 3, 5, 6, 8 and 10. The chromosome 3 QTL qTGW3.2 was stably expressed over two years, and contributed 9-10% of the phenotypic variance. A residual heterozygous line (RHL) was selected from the RIL population and its selfed progeny was used to fine map qTGW3.2. In this "F-2" population, the QTL explained about 23% of the variance, rising to nearly 33% in the subsequent "F-2:3" generation. The physical location of qTGW3.2 was confined to a similar to 556 kb region flanked by the microsatellite loci RM16162 and RM16194. The region also contains other factors influencing certain yield-related traits, although it is also possible that qTGW3.2 affects these in a pleiotropic fashion. Crown Copyright (C) 2013 Published by Elsevier B.V. All rights reserved.