Chemokine-induced secretion of gelatinase B in primary human monocytes
Chemokine-induced secretion of gelatinase B in primary human monocytes
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DOI：
10.1515/bc.2001.173
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发表时间：
2001-09-01
影响因子：
3.7
通讯作者：
Nelson, PJ
中科院分区：
文献类型：
--
作者：
Klier, CM;Nelson, EL;Nelson, PJ
趋化因子有助于控制正常的白细胞运输以及它们在急性和慢性炎症期间向组织中的浸润。基质金属蛋白酶（MMPs）通过对基底膜和基质材料的有限蛋白水解来帮助支持白细胞的外渗和浸润。研究了趋化因子RANTES/CCL 5、MCP-1/CCL和SDF-1 /CXCL 12对基质金属蛋白酶B及其内源性抑制剂TIMP-1分泌的影响。发现RANTES/CCL 5和SDF-1/CXCL 12诱导原代人单核细胞中MMP-9的分泌，而TIMP-1的分泌不受影响。RANTES/CCL 5效应通过CCR 1介导，因为发现CCR 1拮抗剂BX 471有效地阻断RANTES/CCL 5诱导的MMP-9分泌。
Chemokines help control normal leukocyte trafficking as well as their infiltration into tissues during acute and chronic inflammation. Matrix metalloproteinases (MMPs) help support the extravasation and infiltration of leukocytes through limited proteolysis of basement membranes and matrix material. The effect of the chemokines RANTES/CCL5, MCP-1/CCL and SDF-1 /CXCL12 on secretion of the matrix metalloproteinase B and its endogenous inhibitor TIMP-1 was studied. RANTES/CCL5 and SDF-1/CXCL12 were found to induce MMP-9 secretion in primary human monocytes while TIMP-1 secretion was not affected. RANTES/CCL5 effects were mediated through CCR1 because the CCR1 antagonist BX471 was found to effectively block RANTES/CCL5-induced MMP-9 secretion.