Overexpression of the Qc-SNARE gene OsSYP71 enhances tolerance to oxidative stress and resistance to rice blast in rice (Oryza sativa L.)
Overexpression of the Qc-SNARE gene OsSYP71 enhances tolerance to oxidative stress and resistance to rice blast in rice (Oryza sativa L.)
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Qc-SNARE 基因 OsSYP71 的过度表达增强了水稻 (Oryza sativa L.) 对氧化应激的耐受性和对稻瘟病的抵抗力
DOI：
10.1016/j.gene.2012.05.011
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发表时间：
2012-08-10
期刊：
影响因子：
3.5
通讯作者：
Zhang, Hong-Sheng
中科院分区：
文献类型：
--
作者：
Bao, Yong-Mei;Sun, Shu-Jing;Zhang, Hong-Sheng
OsSYP 71是水稻中编码Qc-SNARE蛋白的氧化应激和稻瘟病应答基因。Qc-SNARE蛋白属于SNARE（可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体）超家族，其在真核细胞中作为囊泡运输机制的重要组分起作用。本文从水稻中分离了12个Qc-SNARE基因，并检测了其中9个基因在不同组织中以及在氧化胁迫和稻瘟病胁迫下的表达模式。OsSYP 71基因的表达在胁迫下明显上调。过量表达OsSYP 71的水稻比野生型植株更能耐受氧化胁迫和抗稻瘟病。这些结果表明，Qc-SNARE在水稻对环境胁迫的响应中起重要作用，并且OsSYP 71可用于工程改造具有增强的对氧化胁迫的耐受性和对稻瘟病的抗性的作物。(c)2012爱思唯尔有限公司版权所有。
OsSYP71 is an oxidative stress and rice blast response gene that encodes a Qc-SNARE protein in rice. Qc-SNARE proteins belong to the superfamily of SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors), which function as important components of the vesicle trafficking machinery in eukaryotic cells. In this paper, 12 Qc-SNARE genes were isolated from rice, and expression patterns of 9 genes were detected in various tissues and in seedlings challenged with oxidative stresses and inoculated with rice blast. The expression of OsSYP71 was clearly up-regulated under these stresses. Overexpression of OsSYP71 in rice showed more tolerance to oxidative stress and resistance to rice blast than wild-type plants. These results indicate that Qc-SNAREs play an important role in rice response to environmental stresses, and OsSYP71 is useful in engineering crop plants with enhanced tolerance to oxidative stress and resistance to rice blast. (c) 2012 Elsevier B.V. All rights reserved.