A comparison in vitro of fibroblast attachment to resected root-ends
A comparison in vitro of fibroblast attachment to resected root-ends
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DOI：
10.1046/j.1365-2591.1999.00241.x
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发表时间：
1999-11-01
影响因子：
5
通讯作者：
Bartold, PM
中科院分区：
文献类型：
--
作者：
Weston, GD;Moule, AJ;Bartold, PM
目的探讨不同车针形态对成纤维细胞附着的影响。根管预备后，以AH-26为充填剂，用热塑牙胶充填。将牙根随机分为8个不同的组，并使用8种不同的器械进行根尖根端切除术，其中包括高速和低速布尔斯和手术刀片。在每个牙根被切除后，测量牙根端的表面积。培养的人牙周膜成纤维细胞进行放射性标记，然后允许附着，在体外，根端。通过液体闪烁系统计数确定细胞在切除的根尖上的附着，并表示为每分钟/mm（2）的衰变数。结果成纤维细胞在不同器械预备的根端的附着情况无显著性差异。根端切除对成纤维细胞的初始附着影响很小，因此对根端切除后的愈合影响很小。
Aim The aim of this study was to investigate the effect of surface morphology of root ends resected with various bur configurations on fibroblast attachment.Methodology Seventy-two human single-rooted teeth were collected and decoronated. The root canals were instrumented, and then obturated with thermoplasticized gutta-percha using AH-26 as the sealer. The roots were randomly divided into eight different groups, and apical root-end resections were performed using eight different instruments, which included high and low speed burs and a scalpel blade. After each root was resected, the surface area of the root end was measured. Cultured human periodontal ligament fibroblasts were radiolabelled and then allowed to attach, in vitro, to the root-ends. Cell attachment to the resected root-ends was determined by counting in a liquid scintillation system and expressed as the number of decays per min/mm(2) (DPM/mm(2)) of root surface.Results There was no significant difference in fibroblast attachment to the root-ends prepared with various instruments.Conclusions These findings indicate that the choice of instrument for root-end resection has little influence on the initial attachment of fibroblasts, and thus may have little effect on healing following root-end resection.