A NOVEL STRESS-INDUCIBLE METALLOTHIONEIN-LIKE GENE FROM RICE
A NOVEL STRESS-INDUCIBLE METALLOTHIONEIN-LIKE GENE FROM RICE
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DOI：
10.1007/bf00020388
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发表时间：
1995-06-01
影响因子：
5.1
通讯作者：
HUANG, PC
中科院分区：
文献类型：
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作者：
HSIEH, HM;LIU, WK;HUANG, PC
从水稻基因组文库中克隆了一个新的水稻基因组序列，该序列编码与其他金属硫蛋白类基因同源的编码片段。该序列由两个外显子和一个内含子组成，在此命名为rgMT（水稻基因组金属硫蛋白样基因）。从编码序列预测，rgMT编码一个74个氨基酸的蛋白质。rgMT在水稻植株中的差异表达被观察到，因为成熟转录物在根中比在叶和鞘中更丰富。在不同的胁迫条件下，如过量重金属和热休克，rgMT的表达显着增加。这在250 μ M CuCl2处理16小时、40 ℃加热2小时和0.06%DMSO处理1小时时尤其明显。在蔗糖饥饿条件下，rgMT转录物也随时间增加，直至72 h。在恢复蔗糖饥饿，成绩单略有下降，在12小时内恢复。还观察到rgMT转录物在衰老叶中具有增加的表达。这些结果支持了水稻中rgMT是一个迄今未见报道的胁迫诱导基因的观点。
A novel rice genomic sequence encoding coding segments homologous to other metallothionein-like genes was isolated from Oryza sativa genomic library. This sequence, hereby designated as rgMT (rice genomic metaliothionein-like gene), consists of two exons and one intron. From the coding sequence, it is predicted that rgMT encodes one protein of 74 amino acids. Differential expression of rgMT in rice plants was observed as mature transcripts were more abundant in roots than in leaves and sheaths. Under different stress conditions, such as excess heavy metals and heat shock, expression of rgMT was significantly elevated. This was especially noticeable with 250 mu M CuCl2 for 16 h, 40 degrees C heat for 2 h and 0.06% DMSO for 1 h. Under sucrose starvation, rgMT transcripts also increased with time up to 72 h. During recovery from sucrose starvation, the transcripts declined slightly within 12 h of recovery. rgMT transcripts were also seen to have increased expression in senescent leaves. These results support the notion that rgMT is a stress-inducible gene in rice heretofore unreported.